Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 627559..628358 Replicon chromosome
Accession NZ_CP061764
Organism Escherichia coli O19:H7 strain 730V1

Toxin (Protein)


Gene name yhaV Uniprot ID S1NYM6
Locus tag ID627_RS03040 Protein ID WP_000347251.1
Coordinates 627559..628023 (-) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag ID627_RS03045 Protein ID WP_001307405.1
Coordinates 628023..628358 (-) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ID627_RS03010 622560..622994 - 435 WP_000948824.1 PTS sugar transporter subunit IIA -
ID627_RS03015 623012..623890 - 879 WP_001295548.1 PTS system mannose/fructose/sorbose family transporter subunit IID -
ID627_RS03020 623880..624659 - 780 WP_000406214.1 PTS mannose/fructose/sorbose/N-acetylgalactosamine transporter subunit IIC -
ID627_RS03025 624670..625143 - 474 WP_001295547.1 PTS N-acetylgalactosamine transporter subunit IIB -
ID627_RS03030 625166..626446 - 1281 WP_001547797.1 tagatose-bisphosphate aldolase subunit KbaZ -
ID627_RS03035 626695..627504 + 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
ID627_RS03040 627559..628023 - 465 WP_000347251.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
ID627_RS03045 628023..628358 - 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
ID627_RS03050 628507..630078 - 1572 WP_001273741.1 galactarate dehydratase -
ID627_RS03055 630453..631787 + 1335 WP_000599636.1 galactarate/glucarate/glycerate transporter GarP -
ID627_RS03060 631803..632573 + 771 WP_001058220.1 2-dehydro-3-deoxyglucarate aldolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17777.14 Da        Isoelectric Point: 9.4947

>T172952 WP_000347251.1 NZ_CP061764:c628023-627559 [Escherichia coli O19:H7]
MDFPQRVNGWALYAHPCFQETYDALVAEVEALKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEENH

Download         Length: 465 bp

>T172952 NZ_CP080056:c4637062-4636751 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTTTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT172952 WP_001307405.1 NZ_CP061764:c628358-628023 [Escherichia coli O19:H7]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT172952 NZ_CP080056:c4636750-4636460 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
GCGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCTGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CJ20

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References