Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 3767110..3767651 Replicon chromosome
Accession NZ_CP061758
Organism Escherichia coli O68:H12 strain 97.3

Toxin (Protein)


Gene name yafQ Uniprot ID Q47149
Locus tag IEC98_RS18315 Protein ID WP_000615983.1
Coordinates 3767373..3767651 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID Q47150
Locus tag IEC98_RS18310 Protein ID WP_000729703.1
Coordinates 3767110..3767370 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IEC98_RS18290 3762785..3763570 - 786 WP_000207587.1 putative lateral flagellar export/assembly protein LafU -
IEC98_RS18295 3763542..3765254 + 1713 Protein_3598 flagellar biosynthesis protein FlhA -
IEC98_RS18300 3765478..3765975 - 498 WP_000006255.1 REP-associated tyrosine transposase RayT -
IEC98_RS18305 3766151..3766900 - 750 WP_000056849.1 C40 family peptidase -
IEC98_RS18310 3767110..3767370 + 261 WP_000729703.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
IEC98_RS18315 3767373..3767651 + 279 WP_000615983.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
IEC98_RS18320 3767807..3768547 + 741 WP_001225679.1 murein L,D-transpeptidase -
IEC98_RS18325 3768518..3769285 - 768 WP_000333380.1 class II glutamine amidotransferase -
IEC98_RS18330 3769491..3770069 - 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(4-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-85)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10846.62 Da        Isoelectric Point: 9.9941

>T172947 WP_000615983.1 NZ_CP061758:3767373-3767651 [Escherichia coli O68:H12]
MIQRDIEYSGQYSKDVKLAQKRHKDMNKLKYLMTLLINNTLPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T172947 NZ_CP080056:3435606-3435824 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTTTGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9405.80 Da        Isoelectric Point: 5.0758

>AT172947 WP_000729703.1 NZ_CP061758:3767110-3767370 [Escherichia coli O68:H12]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGIDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT172947 NZ_CP080056:3435206-3435580 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4Q2U

Antitoxin

Source ID Structure
PDB 4Q2U
AlphaFold DB A0A5F1F716

References