Detailed information of TA system TA172946

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 3760913..3761607
• Replicon: chromosome
• Accession: NZ_CP061758
• Organism: Escherichia coli O68:H12 strain 97.3

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: S1EXB8
• Locus tag: IEC98_RS18275
• Protein ID: WP_001263493.1
• Coordinates: 3760913..3761311 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1FJN6
• Locus tag: IEC98_RS18280
• Protein ID: WP_000554757.1
• Coordinates: 3761314..3761607 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IEC98_RS18245	3755913..3757157	-	1245	WP_000189541.1	esterase FrsA	-
-	3756573..3756653	-	81	NuclAT_8	-	-
-	3756573..3756653	-	81	NuclAT_8	-	-
-	3756573..3756653	-	81	NuclAT_8	-	-
-	3756573..3756653	-	81	NuclAT_8	-	-
IEC98_RS18250	3757249..3757707	-	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
IEC98_RS18255	3757968..3759425	+	1458	WP_001293003.1	cytosol nonspecific dipeptidase	-
IEC98_RS18260	3759482..3760003	-	522	Protein_3591	peptide chain release factor H	-
IEC98_RS18265	3760002..3760205	-	204	Protein_3592	RNA ligase RtcB family protein	-
IEC98_RS18270	3760451..3760903	-	453	WP_001059847.1	GNAT family N-acetyltransferase	-
IEC98_RS18275	3760913..3761311	-	399	WP_001263493.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
IEC98_RS18280	3761314..3761607	-	294	WP_000554757.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
IEC98_RS18285	3761659..3762714	-	1056	WP_001226164.1	DNA polymerase IV	-
IEC98_RS18290	3762785..3763570	-	786	WP_000207587.1	putative lateral flagellar export/assembly protein LafU	-
IEC98_RS18295	3763542..3765254	+	1713	Protein_3598	flagellar biosynthesis protein FlhA	-
IEC98_RS18300	3765478..3765975	-	498	WP_000006255.1	REP-associated tyrosine transposase RayT	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15472.83 Da
• Isoelectric Point: 8.0949

>T172946 WP_001263493.1 NZ_CP061758:c3761311-3760913 [Escherichia coli O68:H12]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKQARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T172946 NZ_CP080056:c3407751-3407449 [Escherichia coli]
ATGGCTCAAAAGAATAATATAAGGAGTTTTCGTGATGCCTGGCTGGCTGATTTTTTTGTCCATTCAACGCCACATCGAAA
GATCCCTGCTGAAATCCATACCTCGTTATCACGAAAGTTGGACATCATTAATGCTGCCACTTCTCATCGGGATTTACGCT
CCCCTCCGGGGAACCGATACGAAGAGTTGTCCGGGAAATTACAGGAATATTCCTCCATTAGAGTGAATAAACAGTACCGA
CTGATATTTAAATGGGTTAATGGCAAGGCCGAAGAATTGTTTTTAGACCCACACAACTACTAA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.81 Da
• Isoelectric Point: 5.9050

>AT172946 WP_000554757.1 NZ_CP061758:c3761607-3761314 [Escherichia coli O68:H12]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT172946 NZ_CP080056:c3407413-3407072 [Escherichia coli]
ATGAAACAGGCAACAAGAAAACCGACGACACCCGGAGATATTCTTCTCTATGAATATCTGGAACCGCTCGATTTGAAAAT
CAATGAGTTAGCAGAGTTGCTGCATGTTCATCGTAATAGCGTCAGTGCACTGATCAATAACAATCGTAAACTCACCACTG
AGATGGCATTCCGTCTGGCGAAAGTTTTTGATACCACAGTCGATTTTTGGCTAAACCTCCAGGCGGCAGTTGATCTTTGG
GAAGTTGAAAACAACATGCGTACCCAGGAAGAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGA
GCGTGCAAAAAAGGTCGCGTAG

Structures

Toxin

• AlphaFold DB: S1EXB8

Antitoxin

• AlphaFold DB: S1FJN6