Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 737003..737730 Replicon chromosome
Accession NZ_CP061758
Organism Escherichia coli O68:H12 strain 97.3

Toxin (Protein)


Gene name higB Uniprot ID U9ZMR4
Locus tag IEC98_RS03520 Protein ID WP_000550189.1
Coordinates 737003..737317 (+) Length 105 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag IEC98_RS03525 Protein ID WP_000560266.1
Coordinates 737314..737730 (+) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IEC98_RS03500 733161..734147 - 987 WP_000617698.1 Gfo/Idh/MocA family oxidoreductase -
IEC98_RS03505 734226..734918 - 693 WP_191115599.1 vancomycin high temperature exclusion protein -
IEC98_RS03510 734995..735498 - 504 WP_001333820.1 M48 family metallopeptidase -
IEC98_RS03515 735583..736719 + 1137 WP_000018695.1 23S rRNA (guanine(1835)-N(2))-methyltransferase RlmG -
IEC98_RS03520 737003..737317 + 315 WP_000550189.1 type II toxin-antitoxin system toxin HigB Toxin
IEC98_RS03525 737314..737730 + 417 WP_000560266.1 type II toxin-antitoxin system antitoxin HigA Antitoxin
IEC98_RS03530 737775..739793 - 2019 WP_000121433.1 NADPH-dependent 2,4-dienoyl-CoA reductase -
IEC98_RS03535 740019..742370 - 2352 WP_024238710.1 alpha-glucosidase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(20-92)

Antitoxin

Cro/C1-type helix-turn-helix domain

(84-135)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 12103.10 Da        Isoelectric Point: 10.0409

>T172930 WP_000550189.1 NZ_CP061758:737003-737317 [Escherichia coli O68:H12]
MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAVFPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKC
YIREVMTHKEYDFFTAVHRTKGKK

Download         Length: 315 bp

>T172930 NZ_CP080029:c5506562-5506299 [Streptomyces anulatus]
GTGAGTGAGTACCGCGCCGTCTTCCATCCGGAAGCCCGAGCCGAACTCCGCAAGGTCCCGCGCGACGCCGCACTCCGCAT
CCTGACCAAACTGGCGGAACTGGAAGCCGACCCCTTCGGCTTCAGTACCACTGCGCTCGTTTCCCAGCCCGAGCGCCGCC
GCCTCAGAGTCGGTGACTACCGCGTCATCTACACCGTCGACAACGGCGAACTGATCGTGTGGGTCATTCGCGTCGGACAC
CGATCCACGGTCTACGACAGCTGA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 14995.42 Da        Isoelectric Point: 4.4547

>AT172930 WP_000560266.1 NZ_CP061758:737314-737730 [Escherichia coli O68:H12]
MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLNDPENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGG
IAVIRTLMDQYGLTLSDLPEIGSKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID

Download         Length: 417 bp

>AT172930 NZ_CP080029:c5506821-5506555 [Streptomyces anulatus]
ATGACGCAGATGCCCATAGAGTCCATCCGCGACGTCCGCGCGCACTTGGCCGAGGTCGTCGAACGCGCCGATCGCGACGA
CACACCCACAATCATCACGCGACGAGGCAAAGAAGTCGCCGCCGTCGTGTCCATCGAGGTGCTCCGTAAGTACCAGGAAT
GGGAAGAGCGCGAGATCAACCGCCTCATCGACGAGCGCATGGCGTCCCGGTCTGCCGGCATCCCGATCGAGGACGTCATG
AAGGAGACGCTGGCTCGAAGTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6KMQ
PDB 5IFG
PDB 5YCL
PDB 6KML

Antitoxin

Source ID Structure

References