Detailed information of TA system TA172924

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 3795361..3796055
• Replicon: chromosome
• Accession: NZ_CP061754
• Organism: Escherichia coli O18ac:H14 strain 873.10

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: S1EXB8
• Locus tag: IEC99_RS18500
• Protein ID: WP_001263493.1
• Coordinates: 3795361..3795759 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1FJN6
• Locus tag: IEC99_RS18505
• Protein ID: WP_000554757.1
• Coordinates: 3795762..3796055 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IEC99_RS18470	3790361..3791605	-	1245	WP_044864403.1	esterase FrsA	-
-	3791021..3791101	-	81	NuclAT_10	-	-
-	3791021..3791101	-	81	NuclAT_10	-	-
-	3791021..3791101	-	81	NuclAT_10	-	-
-	3791021..3791101	-	81	NuclAT_10	-	-
IEC99_RS18475	3791697..3792155	-	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
IEC99_RS18480	3792416..3793873	+	1458	WP_001293003.1	cytosol nonspecific dipeptidase	-
IEC99_RS18485	3793930..3794451	-	522	Protein_3642	peptide chain release factor H	-
IEC99_RS18490	3794450..3794653	-	204	Protein_3643	RNA ligase RtcB family protein	-
IEC99_RS18495	3794899..3795351	-	453	WP_001059847.1	GNAT family N-acetyltransferase	-
IEC99_RS18500	3795361..3795759	-	399	WP_001263493.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
IEC99_RS18505	3795762..3796055	-	294	WP_000554757.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
IEC99_RS18510	3796107..3797162	-	1056	WP_001226164.1	DNA polymerase IV	-
IEC99_RS18515	3797233..3798156	-	924	WP_001232547.1	putative lateral flagellar export/assembly protein LafU	-
IEC99_RS18520	3798159..3799022	-	864	WP_001169518.1	flagellar motor stator protein MotA	-
IEC99_RS18525	3799035..3799751	-	717	WP_000938723.1	FliA/WhiG family RNA polymerase sigma factor	-
IEC99_RS18530	3799771..3800238	-	468	WP_000725257.1	flagellar basal body-associated FliL family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15472.83 Da
• Isoelectric Point: 8.0949

>T172924 WP_001263493.1 NZ_CP061754:c3795759-3795361 [Escherichia coli O18ac:H14]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKQARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T172924 NZ_CP080013:c1027424-1027152 [Macrococcus caseolyticus]
ATGCCTAAGAAAAAGATACTATTTTATTCAAGTGGTTTTGAAGATTATACGTACTGGCAAACTAAAGATAAAACTGTATC
AAAGAAAATAAATAAACTAATAAAGAGTATTATGATTGATGGTGCAAATGTAGGTATAGGTAAGCCAGAAAAATTATTAG
GAAACTATACAGGTTGCTATAGCAGAAGGATCACTCATGAACATAGGCTCGTATATAAGATAGAAGATGATTCCATTTGT
ATTTTACAATGTCGTTTTCATTATAAAAGGTAG

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.81 Da
• Isoelectric Point: 5.9050

>AT172924 WP_000554757.1 NZ_CP061754:c3796055-3795762 [Escherichia coli O18ac:H14]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT172924 NZ_CP080013:c1027674-1027417 [Macrococcus caseolyticus]
ATGAAAGTCACAACTTATTCTAATGCAAGATCAAATTTTAGAAAAATAATAGATATTGTTAATGAAGACTGTGAACCTTA
CACAATCACAACAAATACGCATAATGCAGTGATATTATCTGAGCATGACTATAATAGTATGATTGAAACACTTTACTTAC
AAAGTACACCTGCGAATGCCGAAAGATTACAAAAAGCGATTGATGAATCAATTGATAAAAATAACTTACTTCAAGTTGAG
ATTGATTTAGATGCCTAA

Structures

Toxin

• AlphaFold DB: S1EXB8

Antitoxin

• AlphaFold DB: S1FJN6