Detailed information of TA system TA172906

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 76291..77018
• Replicon: plasmid unnamed2
• Accession: NZ_CP061751
• Organism: Escherichia coli O15:H12 strain 2273-PO3

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A663AUB1
• Locus tag: IED00_RS25435
• Protein ID: WP_000558568.1
• Coordinates: 76707..77018 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: V0AHC4
• Locus tag: IED00_RS25430
• Protein ID: WP_000990392.1
• Coordinates: 76291..76710 (-)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IED00_RS25400	71978..72340	-	363	WP_000783215.1	arsenite efflux transporter metallochaperone ArsD	-
IED00_RS25405	72388..72741	-	354	WP_001114073.1	As(III)-sensing metalloregulatory transcriptional repressor ArsR	-
IED00_RS25410	73057..73650	+	594	WP_042111017.1	hypothetical protein	-
IED00_RS25415	74074..74733	+	660	WP_000078540.1	hypothetical protein	-
IED00_RS25420	74730..75059	+	330	WP_000121260.1	hypothetical protein	-
IED00_RS25425	75052..76254	+	1203	WP_000753105.1	hypothetical protein	-
IED00_RS25430	76291..76710	-	420	WP_000990392.1	helix-turn-helix domain-containing protein	Antitoxin
IED00_RS25435	76707..77018	-	312	WP_000558568.1	type II toxin-antitoxin system HigB family toxin	Toxin
IED00_RS25440	77229..77609	+	381	Protein_90	gluconate permease	-
IED00_RS25445	77659..78354	-	696	WP_000370412.1	lactate utilization protein C	-
IED00_RS25450	78347..79774	-	1428	WP_000023895.1	iron-sulfur cluster-binding protein	-
IED00_RS25455	79785..80504	-	720	WP_001102107.1	(Fe-S)-binding protein	-
IED00_RS25460	80568..81265	-	698	Protein_94	IS1 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..96198	96198

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (20-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (83-136)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12372.11 Da
• Isoelectric Point: 10.0935

>T172906 WP_000558568.1 NZ_CP061751:c77018-76707 [Escherichia coli O15:H12]
MHVVSRAPFDTATRQFPNQAAALDDVYRTLKRENYTSPDEMKKRFASLDRMKYREKWWVIDVGGGNLRVMFFADFERGKI
FIKHITTHAEYDKLTDFYRRTKE

Nucleotide

• Length: 312 bp

>T172906 NZ_CP079993:c4193997-4193683 [Escherichia coli]
ATGCACCTGATAACTCAAAAAGCATTGAAAGATGCTGCGGAAAAATACCCGCAACATAAAACGGAGTTGGTGGCTCTGGG
GAACACGATTGCTAAGGGATATTTCAAAAAACCTGAGTCATTAAAAGCAGTATTCCCATCTCTGGATAACTTCAAATATC
TGGATAAGCATTATGTTTTCAATGTTGGGGGCAATGAATTACGTGTTGTAGCAATGGTCTTTTTTGAATCGCAAAAGTGC
TACATACGTGAAGTTATGACGCATAAAGAATACGATTTCTTTACCGCTGTTCATCGTACTAAGGGGAAAAAATGA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15534.71 Da
• Isoelectric Point: 4.4702

>AT172906 WP_000990392.1 NZ_CP061751:c76710-76291 [Escherichia coli O15:H12]
MMYTDAIQAANSLVSIVPLLGGNASRKDYEDALTLVEYLVEHEPDHPLVDMLVAKIAQYEDEAEEFAEFNDRIAALPSGV
ALLRVLMDQHKLTQSDFEEEIGKKSLVSRILNGTRSLTLDHMKALARRFNIPPSSFMDA

Nucleotide

• Length: 420 bp

>AT172906 NZ_CP079993:c4193686-4193270 [Escherichia coli]
ATGATTGCGATTGCCGACATCTTGCAAGCAGGAGAAAAGCTAACTGCTGTGGCACCTTTTCTGGCGGGTATTCAGAACGA
GGAACAATACACCCAGGCGCTGGAACTGGTAGATCATCTGCTGCTCAACGATCCTGAAAACCCCTTGCTGGATCTGGTGT
GTGCCAAAATAACCGCGTGGGAAGAATCAGCGCCCGAATTTGCGGAATTTAATGCCATGGCTCAAGCCATGCCTGGCGGT
ATAGCCGTGATTCGTACCCTTATGGATCAATATGGTTTAACCCTTTCCGATCTGCCGGAAATTGGCAGTAAATCTATGGT
GTCACGCGTTTTGAGCGGGAAGAGGAAATTAACGCTGGAACACGCTAAAAAATTGGCAACGCGATTCGGCATTTCTCCCG
CCTTGTTTATTGATTAA

Structures

Toxin

• AlphaFold DB: A0A663AUB1

Antitoxin

• AlphaFold DB: V0AHC4