Detailed information of TA system TA172903

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 90472..90741
• Replicon: plasmid unnamed1
• Accession: NZ_CP061750
• Organism: Escherichia coli O15:H12 strain 2273-PO3

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: IED00_RS24780
• Protein ID: WP_001312861.1
• Coordinates: 90583..90741 (+)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	90472..90537 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IED00_RS24745	86009..86215	+	207	WP_000547971.1	hypothetical protein	-
IED00_RS24750	86241..86780	+	540	WP_000290812.1	single-stranded DNA-binding protein	-
IED00_RS24755	86842..87075	+	234	WP_000006030.1	DUF905 family protein	-
IED00_RS24760	87140..89098	+	1959	WP_000117210.1	ParB/RepB/Spo0J family partition protein	-
IED00_RS24765	89153..89587	+	435	WP_000845873.1	conjugation system SOS inhibitor PsiB	-
IED00_RS24770	89584..90303	+	720	WP_000116348.1	plasmid SOS inhibition protein A	-
IED00_RS24775	90315..90503	-	189	WP_001299721.1	hypothetical protein	-
-	90315..90539	+	225	NuclAT_0	-	-
-	90315..90539	+	225	NuclAT_0	-	-
-	90315..90539	+	225	NuclAT_0	-	-
-	90315..90539	+	225	NuclAT_0	-	-
-	90472..90537	-	66	-	-	Antitoxin
IED00_RS24780	90583..90741	+	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
IED00_RS24785	91432..91638	+	207	WP_000547971.1	hypothetical protein	-
IED00_RS24790	91663..91950	+	288	WP_000107535.1	hypothetical protein	-
IED00_RS24795	92070..92891	+	822	WP_001234469.1	DUF945 domain-containing protein	-
IED00_RS24800	93186..93776	-	591	WP_165850686.1	transglycosylase SLT domain-containing protein	-
IED00_RS24805	94109..94492	+	384	WP_001151524.1	relaxosome protein TraM	-
IED00_RS24810	94679..95368	+	690	WP_000283380.1	conjugal transfer transcriptional regulator TraJ	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	mrkB / mrkC / mrkD / mrkF	1..129153	129153

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T172903 WP_001312861.1 NZ_CP061750:90583-90741 [Escherichia coli O15:H12]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T172903 NZ_CP079993:c3741476-3741141 [Escherichia coli]
ATGGTAAGCCGATACGTACCCGATACGGGCGATCTGATTTGGGTTGATTTTGACCCGACAAAAGGTAGCGAGCAAGCCGG
ACATCGTCCGGCTGTTGTCCTGAGTCCGTTCATGTACAACAACAAAACAGGTATGTGTCTGTGTGTTCCTTGTACAACGC
AATCAAAAGGATATCCGTTCGAAGTTGTTCTATCCGGTCAGGAACGTGATGGCGTAGCGTTAGCTGATCAGGTAAAAAGT
ATCGCCTGGCGGGCAAGAGGAGCAACGAAGAAAGGAACAGTTGCCCCAGAGGAATTACAACTCATTAAAGCCAAAATTAA
CGTGCTGATTGGGTAG

Antitoxin

Nucleotide

• Length: 66 bp

>AT172903 NZ_CP061750:c90537-90472 [Escherichia coli O15:H12]
GTGGACTAGACATAGGGATGCCTCGTGGTGGTTAATGAAAATTAACTTACTACGGGGCTATCTTCT

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin