Detailed information of TA system TA172884

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): ralRA/-
• Location: 2479204..2479575
• Replicon: chromosome
• Accession: NZ_CP061749
• Organism: Escherichia coli O15:H12 strain 2273-PO3

Toxin (Protein)

• Gene name: ralR
• Uniprot ID: F4VC37
• Locus tag: IED00_RS11950
• Protein ID: WP_001317028.1
• Coordinates: 2479381..2479575 (-)
• Length: 65 a.a.

Antitoxin (RNA)

Gene name	ralA
Locus tag	-
Coordinates	2479204..2479382 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IED00_RS11920	2474382..2475755	+	1374	WP_000123745.1	ATP-dependent RNA helicase DbpA	-
IED00_RS11925	2475884..2476819	-	936	WP_001157407.1	tRNA 2-thiocytidine(32) synthetase TtcA	-
IED00_RS11930	2476871..2478106	-	1236	WP_000040852.1	site-specific integrase	-
IED00_RS11935	2478203..2478900	+	698	Protein_2354	IS1 family transposase	-
IED00_RS11940	2478891..2479100	-	210	WP_097719230.1	excisionase family protein	-
IED00_RS11945	2479179..2479388	-	210	WP_000276809.1	double-strand break reduction protein RcbA	-
-	2479204..2479382	+	179	NuclAT_0	-	Antitoxin
-	2479204..2479382	+	179	NuclAT_0	-	Antitoxin
-	2479204..2479382	+	179	NuclAT_0	-	Antitoxin
-	2479204..2479382	+	179	NuclAT_0	-	Antitoxin
IED00_RS11950	2479381..2479575	-	195	WP_001317028.1	type I toxin-antitoxin system endodeoxyribonuclease toxin RalR	Toxin
IED00_RS11955	2479632..2480441	-	810	WP_000166319.1	recombination protein RecT	-
IED00_RS11960	2480434..2483034	-	2601	WP_001549183.1	exodeoxyribonuclease VIII	-
IED00_RS11965	2483136..2483411	-	276	WP_000632297.1	protein RacC	-
IED00_RS11970	2483486..2483656	-	171	WP_001352098.1	conserved protein, Rac prophage	-
IED00_RS11975	2483656..2483877	-	222	WP_000560223.1	killing protein KilR	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	2455745..2529765	74020
	-	flank	IS/Tn	-	-	2478397..2478900	503

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Endodeoxyribonuclease toxin RalR (6-54)

Sequences

Toxin

Protein

• Length: 65 a.a.
• Molecular weight: 7018.89 Da
• Isoelectric Point: 8.9538

>T172884 WP_001317028.1 NZ_CP061749:c2479575-2479381 [Escherichia coli O15:H12]
MRYDNVKPCPFCGCPSVTVKAISGYYRAKCNGCESRTGYGGSEKEALERWNKRTTGNNNGGVHV

Nucleotide

• Length: 195 bp

>T172884 NZ_CP079993:699053-699403 [Escherichia coli]
ATGGTAAAGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGATCCAGCAAGCGGCCATGAACAGCAAGG
TGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGCCCCCATTACGCAGG
GCGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCGTGGTGCTGGTG
AATCAGGTGCGGATGATGGATCTACACGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGATGAGGTGGTGGAAGAGGC
GTTATTACGCTTGCAGGCGGTGGTGGAATAA

Antitoxin

Nucleotide

• Length: 179 bp

>AT172884 NZ_CP061749:2479204-2479382 [Escherichia coli O15:H12]
GAGGACTGAAGTTTCTCGCAATTAAAATTTATCAATTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGA
GAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTT
CAATAGTGGCGGTAATTTT

Structures

Toxin

• AlphaFold DB: A0A7L6LHV7

Antitoxin