Detailed information of TA system TA172873

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 1168814..1169541
• Replicon: chromosome
• Accession: NZ_CP061749
• Organism: Escherichia coli O15:H12 strain 2273-PO3

Toxin (Protein)

• Gene name: higB
• Uniprot ID: J7Q991
• Locus tag: IED00_RS05675
• Protein ID: WP_000547564.1
• Coordinates: 1168814..1169125 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: IED00_RS05680
• Protein ID: WP_000126294.1
• Coordinates: 1169122..1169541 (+)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IED00_RS05645	1163956..1165665	+	1710	WP_001288140.1	formate hydrogenlyase subunit HycE	-
IED00_RS05650	1165675..1166217	+	543	WP_000493792.1	formate hydrogenlyase subunit HycF	-
IED00_RS05655	1166217..1166984	+	768	WP_000067401.1	formate hydrogenlyase subunit HycG	-
IED00_RS05660	1166981..1167391	+	411	WP_001291918.1	formate hydrogenlyase assembly protein HycH	-
IED00_RS05665	1167384..1167854	+	471	WP_000132961.1	hydrogenase maturation peptidase HycI	-
IED00_RS05670	1167879..1168640	+	762	WP_001026446.1	hypothetical protein	-
IED00_RS05675	1168814..1169125	+	312	WP_000547564.1	type II toxin-antitoxin system HigB family toxin	Toxin
IED00_RS05680	1169122..1169541	+	420	WP_000126294.1	helix-turn-helix domain-containing protein	Antitoxin
IED00_RS05685	1169655..1171079	-	1425	WP_000110363.1	6-phospho-beta-glucosidase AscB	-
IED00_RS05690	1171088..1172545	-	1458	WP_001107882.1	PTS cellobiose/arbutin/salicin transporter subunit IIBC	-
IED00_RS05695	1172805..1173815	+	1011	WP_001402444.1	DNA-binding transcriptional regulator AscG	-
IED00_RS05700	1173964..1174491	+	528	WP_001078777.1	electron transport protein HydN	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12426.13 Da
• Isoelectric Point: 9.7248

>T172873 WP_000547564.1 NZ_CP061749:1168814-1169125 [Escherichia coli O15:H12]
MHIISKAPFEESARKYPNDALALQALYRVIKETDFSTPEEMRTAFPNLDNFRYRNKWYVLDVGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T172873 NZ_CP079992:c2943764-2943510 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGACGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15416.42 Da
• Isoelectric Point: 4.4596

>AT172873 WP_000126294.1 NZ_CP061749:1169122-1169541 [Escherichia coli O15:H12]
MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLASRIAEYENNNEKFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT172873 NZ_CP079992:c2944012-2943761 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGCTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATCATTGAGTGA

Structures

Toxin

• AlphaFold DB: A0A0A0FJG5