Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 708782..709509 Replicon chromosome
Accession NZ_CP061749
Organism Escherichia coli O15:H12 strain 2273-PO3

Toxin (Protein)


Gene name higB Uniprot ID U9ZMR4
Locus tag IED00_RS03465 Protein ID WP_000550189.1
Coordinates 708782..709096 (+) Length 105 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag IED00_RS03470 Protein ID WP_000560266.1
Coordinates 709093..709509 (+) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IED00_RS03445 704947..705933 - 987 WP_001301393.1 Gfo/Idh/MocA family oxidoreductase -
IED00_RS03450 706012..706695 - 684 WP_001183040.1 vancomycin high temperature exclusion protein -
IED00_RS03455 706772..707275 - 504 WP_001295542.1 M48 family metallopeptidase -
IED00_RS03460 707361..708497 + 1137 WP_000018697.1 23S rRNA (guanine(1835)-N(2))-methyltransferase RlmG -
IED00_RS03465 708782..709096 + 315 WP_000550189.1 type II toxin-antitoxin system toxin HigB Toxin
IED00_RS03470 709093..709509 + 417 WP_000560266.1 type II toxin-antitoxin system antitoxin HigA Antitoxin
IED00_RS03475 709554..711572 - 2019 WP_000121421.1 NADPH-dependent 2,4-dienoyl-CoA reductase -
IED00_RS03480 712098..714449 - 2352 WP_000695498.1 alpha-glucosidase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(20-92)

Antitoxin

Cro/C1-type helix-turn-helix domain

(84-135)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 12103.10 Da        Isoelectric Point: 10.0409

>T172867 WP_000550189.1 NZ_CP061749:708782-709096 [Escherichia coli O15:H12]
MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAVFPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKC
YIREVMTHKEYDFFTAVHRTKGKK

Download         Length: 315 bp

>T172867 NZ_CP079992:c2149232-2149125 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGATTTTCTGGCACGACCTGGCAGCACCGATCCTGGCGGGAATTATTACCGCAGCGAT
TGTCAGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 14995.42 Da        Isoelectric Point: 4.4547

>AT172867 WP_000560266.1 NZ_CP061749:709093-709509 [Escherichia coli O15:H12]
MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLNDPENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGG
IAVIRTLMDQYGLTLSDLPEIGSKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID

Download         Length: 417 bp

>AT172867 NZ_CP079992:2149279-2149345 [Escherichia coli]
TGTCTGGTTTCAAGATTAGCCCCCGTTTTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6KMQ
PDB 5IFG
PDB 5YCL
PDB 6KML

Antitoxin

Source ID Structure

References