Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 665404..666203 Replicon chromosome
Accession NZ_CP061749
Organism Escherichia coli O15:H12 strain 2273-PO3

Toxin (Protein)


Gene name yhaV Uniprot ID D7XZ20
Locus tag IED00_RS03245 Protein ID WP_000347264.1
Coordinates 665404..665868 (-) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag IED00_RS03250 Protein ID WP_001307405.1
Coordinates 665868..666203 (-) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IED00_RS03215 660405..660839 - 435 WP_000948824.1 PTS sugar transporter subunit IIA -
IED00_RS03220 660857..661735 - 879 WP_001300474.1 PTS system mannose/fructose/sorbose family transporter subunit IID -
IED00_RS03225 661725..662504 - 780 WP_000406214.1 PTS mannose/fructose/sorbose/N-acetylgalactosamine transporter subunit IIC -
IED00_RS03230 662515..662988 - 474 WP_001295547.1 PTS N-acetylgalactosamine transporter subunit IIB -
IED00_RS03235 663011..664291 - 1281 WP_097719126.1 tagatose-bisphosphate aldolase subunit KbaZ -
IED00_RS03240 664540..665349 + 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
IED00_RS03245 665404..665868 - 465 WP_000347264.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
IED00_RS03250 665868..666203 - 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
IED00_RS03255 666352..667923 - 1572 WP_001273753.1 galactarate dehydratase -
IED00_RS03260 668298..669632 + 1335 WP_000599636.1 galactarate/glucarate/glycerate transporter GarP -
IED00_RS03265 669648..670418 + 771 WP_001058207.1 2-dehydro-3-deoxyglucarate aldolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17894.29 Da        Isoelectric Point: 9.4945

>T172866 WP_000347264.1 NZ_CP061749:c665868-665404 [Escherichia coli O15:H12]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFDAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH

Download         Length: 465 bp

>T172866 NZ_CP079992:c2148697-2148590 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGACTTTCTGGCACGACCTGGCGGCACCGATCCTGGCGGGAATTATTACCGCAGCGAT
TGTCGGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT172866 WP_001307405.1 NZ_CP061749:c666203-665868 [Escherichia coli O15:H12]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT172866 NZ_CP079992:2148744-2148810 [Escherichia coli]
TGTCTGGTTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A241QML2

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References