Detailed information of TA system TA172834

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/RelE-HTH
• Location: 4090..4675
• Replicon: plasmid p4E072658
• Accession: NZ_CP061710
• Organism: Acinetobacter baumannii strain E-072658

Toxin (Protein)

• Gene name: higB
• Uniprot ID: N8ZPD7
• Locus tag: H2787_RS18460
• Protein ID: WP_000897309.1
• Coordinates: 4355..4675 (-)
• Length: 107 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: H2787_RS18455
• Protein ID: WP_004641921.1
• Coordinates: 4090..4362 (-)
• Length: 91 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H2787_RS18435 (H2787_18430)	1..951	+	951	WP_126132568.1	replication initiation protein RepM	-
H2787_RS18440 (H2787_18435)	944..1519	+	576	WP_180075651.1	plasmid replication DNA-binding protein	-
H2787_RS18445 (H2787_18440)	1532..1675	+	144	WP_001125247.1	hypothetical protein	-
H2787_RS18450 (H2787_18445)	1884..3338	-	1455	WP_005401409.1	SulP family inorganic anion transporter	-
H2787_RS18455 (H2787_18450)	4090..4362	-	273	WP_004641921.1	NadS family protein	Antitoxin
H2787_RS18460 (H2787_18455)	4355..4675	-	321	WP_000897309.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
H2787_RS18465 (H2787_18460)	4892..5302	+	411	WP_126132571.1	hypothetical protein	-
H2787_RS18470 (H2787_18465)	5313..5867	+	555	WP_126132572.1	hypothetical protein	-
H2787_RS18475 (H2787_18470)	5864..6277	+	414	WP_126132573.1	hypothetical protein	-
H2787_RS18480 (H2787_18475)	6399..6767	-	369	WP_180073047.1	LysR family transcriptional regulator	-
H2787_RS18485 (H2787_18480)	7073..7414	+	342	WP_223106977.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..7989	7989

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (41-102)

Antitoxin

• Cro/C1-type helix-turn-helix domain (37-85)

Sequences

Toxin

Protein

• Length: 107 a.a.
• Molecular weight: 12209.01 Da
• Isoelectric Point: 7.9835

>T172834 WP_000897309.1 NZ_CP061710:c4675-4355 [Acinetobacter baumannii]
MLFIETSIFTKQIKELVSDEEYRQLQQDLLVQPDKGDLIKNGGGIRKVRCAQGNKGKSGGIRVIYYWVTEDDQIFFLVAY
PKSVKDNLTDKETSILRQLVKEQFHG

Nucleotide

• Length: 321 bp

>T172834 NZ_CP079991:c830201-829860 [Escherichia coli]
ATGACTGACACGCATTCTATTGCACAACCGTTCGAAGCAGAAGTCTCCCCGGCAAATAACCGTCATGTCACCGTCGGTTA
TGCGAGTCGCTACCCGGATTACAGCCGTATTCCCGCCATCACCCTGAAAGGTCAGTGGCTGGAAGCCGCCGGTTTTGCCA
CTGGCACGGCGGTAGATGTCAAAGTGATGGAAGGCTGTATTGTCCTCACCGCCCAACCACCCGCCGCCGAGGAGAGCGAA
CTGATGCAGTCGCTACGCCAGGTGTGCAAACTGTCGGCGCGTAAACAAAAGCAGGTGCAGGCGTTTATTGGAGTGATTGC
CGGTAAACAGAAAGTCGCGTAA

Antitoxin

Protein

• Length: 91 a.a.
• Molecular weight: 10108.56 Da
• Isoelectric Point: 7.2045

>AT172834 WP_004641921.1 NZ_CP061710:c4362-4090 [Acinetobacter baumannii]
MDNNLFDDLVASIKEAGAIKRNEIKASRVTELELPDIKEVREKTGLTQAEFAARLHISARTLQNWEQGRRYPTGPAATLI
RILDAHPTLI

Nucleotide

• Length: 273 bp

>AT172834 NZ_CP079991:830196-830272 [Escherichia coli]
AGTCATAACTGCTATTCTCCAGGAATAGTGATTGTGATTAGCGATGCGGGTGTGTTGGCGCACATCCGCACCGCGCT

Structures

Toxin

• AlphaFold DB: A0A855WJQ3