Detailed information of TA system TA172830

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/RelE-HTH
• Location: 63684..64182
• Replicon: plasmid p8E072658
• Accession: NZ_CP061706
• Organism: Acinetobacter baumannii strain E-072658

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: H2787_RS17895
• Protein ID: WP_223106925.1
• Coordinates: 63949..64182 (-)
• Length: 78 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: H2787_RS17890
• Protein ID: WP_000369781.1
• Coordinates: 63684..63956 (-)
• Length: 91 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H2787_RS17855 (H2787_17850)	58934..59275	-	342	WP_223106961.1	hypothetical protein	-
H2787_RS17860 (H2787_17855)	59590..59958	+	369	WP_223106962.1	LysR family transcriptional regulator	-
H2787_RS17865 (H2787_17860)	60047..60352	-	306	WP_120366367.1	hypothetical protein	-
H2787_RS17870 (H2787_17865)	60596..60919	+	324	WP_000288001.1	hypothetical protein	-
H2787_RS17875 (H2787_17870)	60900..61214	+	315	WP_223106923.1	helix-turn-helix domain-containing protein	-
H2787_RS17880 (H2787_17875)	61269..61586	+	318	WP_223106924.1	hypothetical protein	-
H2787_RS17885 (H2787_17880)	62192..63085	-	894	WP_216972119.1	HNH endonuclease	-
H2787_RS17890 (H2787_17885)	63684..63956	-	273	WP_000369781.1	NadS family protein	Antitoxin
H2787_RS17895 (H2787_17890)	63949..64182	-	234	WP_223106925.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
H2787_RS17900 (H2787_17895)	64257..65243	-	987	WP_004278529.1	IS110 family transposase	-
H2787_RS17905 (H2787_17900)	65484..65714	+	231	Protein_77	transposase domain-containing protein	-
H2787_RS17910 (H2787_17905)	65746..66302	+	557	Protein_78	IS5 family transposase	-
H2787_RS17915 (H2787_17910)	66557..66832	+	276	WP_001133624.1	metal/formaldehyde-sensitive transcriptional repressor	-
H2787_RS17920 (H2787_17915)	66854..67966	+	1113	WP_000842149.1	S-(hydroxymethyl)glutathione dehydrogenase/class III alcohol dehydrogenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	mcr-4.3	-	1..119750	119750

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (12-73)

Antitoxin

• Cro/C1-type helix-turn-helix domain (37-85)

Sequences

Toxin

Protein

• Length: 78 a.a.
• Molecular weight: 8711.02 Da
• Isoelectric Point: 9.8930

>T172830 WP_223106925.1 NZ_CP061706:c64182-63949 [Acinetobacter baumannii]
MVQPDRGDLIKNGGGIRKVRCAQGNKGKSGGIRVIYYWVTEDDQIFFLVAYPKSVKDNLTDKETAILRQLVKEQFHG

Nucleotide

• Length: 234 bp

>T172830 NZ_CP079985:43016-43363 [Macrococcus bohemicus]
TTGGTAGAAGAAGTATCACAAGGTGATATATTTTATGTTAATTTCAATCCAAGAAAAGGTCATGAACAGAAAGATAAGCG
ACCAGCTCTTGCAATCAGTCACGATATGGTAAGAGATACCAGCAATTTAACCATTGTAGTTCCAATATCTAACACTAAAA
GAAGTTTTCCTATGTATCACACATTGAAAACAACAAAAAAGATTGAAGGTAAAGTATTGTTAGATCAATCAATTGCTCTT
GATTTGAAAGCAAGAGGAATATCGAAGGATAATGTAGTTGAAATAATAGACAAAGAAGAACTTAGCGAAATTATACATCT
GTATAAACTATTATTTACGGTAGATTGA

Antitoxin

Protein

• Length: 91 a.a.
• Molecular weight: 10149.61 Da
• Isoelectric Point: 8.6582

>AT172830 WP_000369781.1 NZ_CP061706:c63956-63684 [Acinetobacter baumannii]
MDNNLFDDLVASIKEAGAIKRKEVKASRVTELELPDIKEVREKTGLSQNEFAARLHISPRTLQNWEQGRRYPTGPAATLI
RILDAHPSLI

Nucleotide

• Length: 273 bp

>AT172830 NZ_CP079985:42759-43022 [Macrococcus bohemicus]
ATGAGTACATTAGAAACAACTACAACATTAAAAAAATGGGGAAATTCAAAAGGATTGAGATTACCTCAAAAAGTTATTAC
ACACCTGAATTTACATGAAGATCAAAAGATATTAATAGTTTTAGAAGAAGATAAGTTGATTTTAAAACCACAAACAGAAA
AGCCTACAAATATTCATGAATTATTTTCTGGATGGAAAGATGACGGTATTCGTAGTGAAGAAATGGATTGGGGGCAGGCA
GAAGGTGAGGAATTAAATTGGTAG