Detailed information of TA system TA172829

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/RelE-HTH
• Location: 49075..49660
• Replicon: plasmid p8E072658
• Accession: NZ_CP061706
• Organism: Acinetobacter baumannii strain E-072658

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A385C123
• Locus tag: H2787_RS17785
• Protein ID: WP_058971762.1
• Coordinates: 49340..49660 (-)
• Length: 107 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: H2787_RS17780
• Protein ID: WP_000369781.1
• Coordinates: 49075..49347 (-)
• Length: 91 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H2787_RS17745 (H2787_17740)	45686..46135	+	450	WP_228750196.1	hypothetical protein	-
H2787_RS17750 (H2787_17745)	46282..46491	+	210	WP_228199412.1	hypothetical protein	-
H2787_RS17755 (H2787_17750)	46497..47048	+	552	WP_000755487.1	macro domain-containing protein	-
H2787_RS17760 (H2787_17755)	47050..47436	-	387	WP_000053135.1	type II toxin-antitoxin system death-on-curing family toxin	-
H2787_RS17765 (H2787_17760)	47433..47612	-	180	WP_001231652.1	hypothetical protein	-
H2787_RS17770 (H2787_17765)	47662..47997	-	336	WP_000689753.1	hypothetical protein	-
H2787_RS17775 (H2787_17770)	48053..48934	-	882	WP_004282326.1	IS982-like element ISAba9 family transposase	-
H2787_RS17780 (H2787_17775)	49075..49347	-	273	WP_000369781.1	NadS family protein	Antitoxin
H2787_RS17785 (H2787_17780)	49340..49660	-	321	WP_058971762.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
H2787_RS17790 (H2787_17785)	49827..50087	+	261	WP_068976015.1	hypothetical protein	-
H2787_RS17795 (H2787_17790)	50779..51222	+	444	WP_001109442.1	universal stress protein	-
H2787_RS17800 (H2787_17795)	51449..52273	-	825	WP_070155722.1	IS4 family transposase	-
H2787_RS17805 (H2787_17800)	52598..53215	+	618	WP_223106956.1	DedA family protein	-
H2787_RS17810 (H2787_17805)	53246..53614	+	369	WP_070155725.1	ABZJ_00895 family protein	-
H2787_RS17815 (H2787_17810)	53584..53703	+	120	Protein_59	DUF4372 domain-containing protein	-
H2787_RS17820 (H2787_17815)	53729..53974	-	246	WP_000246756.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	mcr-4.3	-	1..119750	119750
	-	flank	IS/Tn	-	-	48053..48934	881
	-	flank	IS/Tn	-	-	54084..55421	1337

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (41-102)

Antitoxin

• Cro/C1-type helix-turn-helix domain (37-85)

Sequences

Toxin

Protein

• Length: 107 a.a.
• Molecular weight: 12207.98 Da
• Isoelectric Point: 6.9800

>T172829 WP_058971762.1 NZ_CP061706:c49660-49340 [Acinetobacter baumannii]
MLFIETSIFTKQIKDLVSDEEYRQLQEDLLVQPDRGDLIKNGGGIRKVRCAQGNKGKSGGIRVIYYWVTEDDQIFFLVAY
PKSVKDNLTDKETAILRQLVKEQFHG

Nucleotide

• Length: 321 bp

>T172829 NZ_CP079984:c2031895-2031533 [Macrococcus bohemicus]
ATGAAACGAGGCGATGTTTATTTAGCTGATTTGTCACCTGTTAAAGGTTCAGAGCAAGGCGGTAAACGACCTGTAGTAAT
CATTCAAAATGACATAGGAAATAAATATAGTCCGACGGTTATAGTTGCAGCTATCACTGCTAAAATCAATAAATCAAAAA
TCCCAACTCACGTAGAGATTGGTAAGACCAATCATCATCTAGATAAAGATTCTGTGATTTTATTAGAACAAATAAGAACA
ATTGATAAAAATCGACTTATTGAAAAATTGACTTACCTGCCAAAAGAGAAGATGGAGGAAGTTGATGCAGCACTAGCAAT
TAGTCTTAATTTATCTATTGATGACTCGGAGACTTTAGGCTAA

Antitoxin

Protein

• Length: 91 a.a.
• Molecular weight: 10149.61 Da
• Isoelectric Point: 8.6582

>AT172829 WP_000369781.1 NZ_CP061706:c49347-49075 [Acinetobacter baumannii]
MDNNLFDDLVASIKEAGAIKRKEVKASRVTELELPDIKEVREKTGLSQNEFAARLHISPRTLQNWEQGRRYPTGPAATLI
RILDAHPSLI

Nucleotide

• Length: 273 bp

>AT172829 NZ_CP079984:c2032042-2031896 [Macrococcus bohemicus]
ATGAGTAAACTACTAGAACAACAATTGATTGAAGGTTATCAATCAATGGCTGAAATTAATCTGTTAATTGCTTCAGAACA
TTATCTCCTTGAATGTGAAGTGTATGATAATGTGGATCATATGTCAGTAAAAAATGATGGTGAATAA

Structures

Toxin

• AlphaFold DB: A0A385C123