Detailed information of TA system TA172812

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/Tad-HTH_37
• Location: 3848461..3849058
• Replicon: chromosome
• Accession: NZ_CP061700
• Organism: Klebsiella pneumoniae strain C39

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A9J6S5A1
• Locus tag: IE968_RS18770
• Protein ID: WP_004893639.1
• Coordinates: 3848741..3849058 (-)
• Length: 106 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: R4YH91
• Locus tag: IE968_RS18765
• Protein ID: WP_004142561.1
• Coordinates: 3848461..3848748 (-)
• Length: 96 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IE968_RS18735	3844668..3844916	+	249	WP_002893037.1	DUF1158 domain-containing protein	-
IE968_RS18740	3844934..3845275	-	342	WP_002893035.1	RamA family antibiotic efflux transcriptional regulator	-
IE968_RS18745	3845306..3846421	-	1116	WP_074180844.1	MBL fold metallo-hydrolase	-
IE968_RS18750	3846601..3847182	+	582	WP_004176968.1	TetR/AcrR family transcriptional regulator	-
IE968_RS18755	3847182..3847550	+	369	WP_004142557.1	MmcQ/YjbR family DNA-binding protein	-
IE968_RS18760	3847670..3848323	+	654	WP_004178896.1	oxygen-insensitive NAD(P)H nitroreductase	-
IE968_RS18765	3848461..3848748	-	288	WP_004142561.1	helix-turn-helix transcriptional regulator	Antitoxin
IE968_RS18770	3848741..3849058	-	318	WP_004893639.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
IE968_RS18775	3849243..3850286	-	1044	WP_023279204.1	DUF2157 domain-containing protein	-
IE968_RS18780	3850956..3851822	-	867	WP_004151823.1	helix-turn-helix transcriptional regulator	-
IE968_RS18785	3851931..3853358	+	1428	WP_004176980.1	MFS transporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-100)

Antitoxin

• Cro/C1-type helix-turn-helix domain (38-88)

Sequences

Toxin

Protein

• Length: 106 a.a.
• Molecular weight: 12100.35 Da
• Isoelectric Point: 11.2767

>T172812 WP_004893639.1 NZ_CP061700:c3849058-3848741 [Klebsiella pneumoniae]
MFRLVVHVDVKKELQALPSIVQAKMIRQLDKLRQNPTALREPDSKPLGQGLFEIRALGSVQGRGIYVFQQGKTLFLLRVF
VKKTQKTPSSEIRLALKRLEEMRNE

Nucleotide

• Length: 318 bp

>T172812 NZ_CP079940:4718003-4718188 [Achromobacter sp. ES-001]
ATGAAATACAGCGAGCTCAGGCGGTGGCTGCTGAAACAGGGAGTGAAGTTAGAACCCCACAAATCAGGTAGCAGCCACTT
CAAAGCAACCCTTGGCGATCTGCAAACCAGCTTTCCAGACCACGGCAGTAAAGAAATCGGTATGGGGTTGGTGCAGAAGA
TCAAAAAGGATCTTGGACTGAAATAA

Antitoxin

Protein

• Length: 96 a.a.
• Molecular weight: 10771.46 Da
• Isoelectric Point: 10.2212

>AT172812 WP_004142561.1 NZ_CP061700:c3848748-3848461 [Klebsiella pneumoniae]
MSKKIIDWDTLKTELLGQPEVQAAFDAQERQQRLRTMLAQWREQAGLTRAQVAQRMGVSAPTVSRMEANITRASLDTLTR
YALACGIKHPQVLLY

Nucleotide

• Length: 288 bp

>AT172812 NZ_CP079940:4718258-4718671 [Achromobacter sp. ES-001]
ATGTTGACCTACTGCTACACCCTGACGCCGGACACGAACGGCACGAACCTGATTCAGTATCCGGACCTGCCGGAAGGCGC
ATCCGTAAGTGAAGACGAGCACGACCTACAAGCCAACGCATCAGAAGGGCTTGAGGCCGTGCTACAGATGTACATCGACG
CGCGCCGCCCTATCCCGCTGCCTGAAGCCGTCGGCGACGGCAGCGTGACGCTAGGCGCACTGCCTACCGCGAAGGTCTTC
CTGTCAAACGAGATGGTGCGTCAAGGAGTGCGAAAAACCGAACTCGCGCGGCGACTCGGCATCCACAACCCCCAGGTTGA
CCGCCTCCTGGATCTGGCGCACAGTTCCAAGCTGGAAGCGATGGAAGCTGCGTTTCGAGAATTGGGCCGCCGCTTGGACA
TTTCTGTCGCCTGA

Structures

Antitoxin

• AlphaFold DB: A0A0S3DIQ1