Detailed information of TA system TA172789

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 48129..48780
• Replicon: plasmid pAS9-1
• Accession: NZ_CP061682
• Organism: Acinetobacter seifertii strain AS9

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A7H2WXC0
• Locus tag: IC766_RS19115
• Protein ID: WP_006581604.1
• Coordinates: 48424..48780 (-)
• Length: 119 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: IC766_RS19110
• Protein ID: WP_151793077.1
• Coordinates: 48129..48431 (-)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IC766_RS19065	43548..43845	+	298	Protein_47	hypothetical protein	-
IC766_RS19070	43796..44050	+	255	WP_000236586.1	hypothetical protein	-
IC766_RS19075	44129..44418	-	290	Protein_49	helix-turn-helix domain-containing protein	-
IC766_RS19080	44405..44593	-	189	WP_000033474.1	type II toxin-antitoxin system RelE/ParE family toxin	-
IC766_RS19085	44870..45190	-	321	WP_002123990.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
IC766_RS19090	45376..45689	+	314	Protein_52	AAA family ATPase	-
IC766_RS19095	45769..46928	+	1160	WP_085945698.1	IS3 family transposase	-
IC766_RS19100	47599..47817	+	219	WP_029747731.1	hypothetical protein	-
IC766_RS19105	47927..48136	-	210	WP_000069471.1	hypothetical protein	-
IC766_RS19110	48129..48431	-	303	WP_151793077.1	XRE family transcriptional regulator	Antitoxin
IC766_RS19115	48424..48780	-	357	WP_006581604.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
IC766_RS19120	49590..51044	+	1455	WP_062032131.1	SulP family inorganic anion transporter	-
IC766_RS19125	51258..51620	-	363	Protein_59	DNA-binding protein	-
IC766_RS19130	51654..51830	-	177	WP_006581600.1	hypothetical protein	-
IC766_RS19135	52115..53323	+	1209	WP_004832869.1	IS256-like element ISAba26 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..108220	108220
	-	flank	IS/Tn	-	-	52115..53323	1208

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-106)

Antitoxin

• HigA2-like, helix-turn-helix domain (13-89)

Sequences

Toxin

Protein

• Length: 119 a.a.
• Molecular weight: 13462.38 Da
• Isoelectric Point: 7.1642

>T172789 WP_006581604.1 NZ_CP061682:c48780-48424 [Acinetobacter seifertii]
MWTVITTDLFNEWLAQQDQSTQEKVLAALVVLQQQGPSLGRPLVDTVYDSKFTNMKDLRVQHRGKSLRAFFAFDPLRQAI
VLCIGDKGGKKRFYKEMLDIADQQYEIHLSTLGDQSNG

Nucleotide

• Length: 357 bp

>T172789 NZ_CP079926:c969021-968731 [Caulobacter vibrioides]
ATGAAGCCCTATCGGCTATCCCGTCGCGCCAAGGCCGACCTCGATGACATCTGGACCTATTCAGAGCAGCGATGGGGCGT
TGAACAAGCCGCCGACTACGCCCGTGAACTGCAGGCCACCATCGAGATGATCGCTGAACATCCGGGTATGGGACAGCCGG
ATGAAAACCTCCGCGCGGGCTACAGGCGATGCGCCAGCGGCTCGCACGTGGTCTTTTACCGCGTCGGCGTGCGGGTCGAG
ATTATCCGCGTGCTACACCAAAGCATGAACGCCAGGGCTCACCTTGGCTGA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11191.98 Da
• Isoelectric Point: 6.7277

>AT172789 WP_151793077.1 NZ_CP061682:c48431-48129 [Acinetobacter seifertii]
MAKTLQELLANRSPDSQARIQQMADELLLETQLHLIREELEISQKELAATLGIKQPSLSAIENRGHDLKISTMKKYVEAM
GGKLRIDVELPTGKHIGFNV

Nucleotide

• Length: 303 bp

>AT172789 NZ_CP079926:c969284-969018 [Caulobacter vibrioides]
ATGGCGAGCAAGAACACCTCCGTCGTCCTGGGCGATCACTTCCAGGCGTTCATCGACAGCCAGGTCGCTGACGGGCGCTA
CGGCTCGGCCAGCGAAGTCATCCGCGCGGGGCTGAGGCTCCTCGAAGAGAACGAAGCGAAGCTCGCGGCCCTCCGCGCCG
CCCTGATCGAAGGCGAGGAGAGCGGCTTTATCGAGGACTTCGACTTCGACGCCTTTATCGAGGAAAGGTCTAGGGCATCC
GCTCCGCAGGGGTTCCATGAGGAATGA

Structures

Toxin

• AlphaFold DB: A0A7H2WXC0