Detailed information of TA system TA172786

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 46915..47566
• Replicon: plasmid pAS11-1
• Accession: NZ_CP061679
• Organism: Acinetobacter seifertii strain AS11

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A7H2WXC0
• Locus tag: IC767_RS20285
• Protein ID: WP_006581604.1
• Coordinates: 47210..47566 (-)
• Length: 119 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: IC767_RS20280
• Protein ID: WP_001140619.1
• Coordinates: 46915..47217 (-)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IC767_RS20235	42334..42631	+	298	Protein_44	hypothetical protein	-
IC767_RS20240	42582..42836	+	255	WP_000236586.1	hypothetical protein	-
IC767_RS20245	42915..43204	-	290	Protein_46	helix-turn-helix domain-containing protein	-
IC767_RS20250	43191..43379	-	189	WP_000033474.1	type II toxin-antitoxin system RelE/ParE family toxin	-
IC767_RS20255	43656..43976	-	321	WP_002123990.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
IC767_RS20260	44162..44475	+	314	Protein_49	AAA family ATPase	-
IC767_RS20265	44555..45714	+	1160	WP_085945698.1	IS3 family transposase	-
IC767_RS20270	46385..46603	+	219	WP_029747731.1	hypothetical protein	-
IC767_RS20275	46713..46922	-	210	WP_000069471.1	hypothetical protein	-
IC767_RS20280	46915..47217	-	303	WP_001140619.1	XRE family transcriptional regulator	Antitoxin
IC767_RS20285	47210..47566	-	357	WP_006581604.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
IC767_RS20290	48376..49830	+	1455	WP_062032131.1	SulP family inorganic anion transporter	-
IC767_RS20295	50044..50406	-	363	Protein_56	DNA-binding protein	-
IC767_RS20300	50440..50616	-	177	WP_006581600.1	hypothetical protein	-
IC767_RS20305	50901..52109	+	1209	WP_004832869.1	IS256-like element ISAba26 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..105815	105815
	-	flank	IS/Tn	-	-	50901..52109	1208

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-106)

Antitoxin

• HigA2-like, helix-turn-helix domain (13-89)

Sequences

Toxin

Protein

• Length: 119 a.a.
• Molecular weight: 13462.38 Da
• Isoelectric Point: 7.1642

>T172786 WP_006581604.1 NZ_CP061679:c47566-47210 [Acinetobacter seifertii]
MWTVITTDLFNEWLAQQDQSTQEKVLAALVVLQQQGPSLGRPLVDTVYDSKFTNMKDLRVQHRGKSLRAFFAFDPLRQAI
VLCIGDKGGKKRFYKEMLDIADQQYEIHLSTLGDQSNG

Nucleotide

• Length: 357 bp

>T172786 NZ_CP079924:c4032866-4032516 [Escherichia sp. TC-EC600-tetX4]
ATGGTAAAGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGATCCAGCAAGCGGCCATGAACAGCAAGG
TGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGCCCCCATTACGCAGG
GCGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCGTGGTGCTGGTG
AATCAGGTGCGGATGATGGATCTACACGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGATGAGGTGGTGGAAGAGGC
GTTATTACGCTTGCAGGCGGTGGTGGAATAA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11206.01 Da
• Isoelectric Point: 6.7282

>AT172786 WP_001140619.1 NZ_CP061679:c47217-46915 [Acinetobacter seifertii]
MAKTLQELLANRSPESQARIQQMADELLLETQLHLIREELEISQKELAATLGIKQPSLSAIENRGHDLKISTMKKYVEAM
GGKLRIDVELPTGKHIGFNV

Nucleotide

• Length: 303 bp

>AT172786 NZ_CP079924:c4033111-4032860 [Escherichia sp. TC-EC600-tetX4]
ATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTACA
GCCGGGGCAGAGCGTGGAGGCGCAAGTGAGCAACAATCAACTGATTCTGACACCCATCTCCAGGCGCTACTCGCTTGATG
AACTGCTGGCACAGTGTGACATGAACGCCGCGGAACTTAGCGAGCAGGATGTCTGGGGTAAATCCACCCCTGCGGGTGAC
GAAATATGGTAA

Structures

Toxin

• AlphaFold DB: A0A7H2WXC0