Detailed information of TA system TA172784

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 64652..65310
• Replicon: plasmid pAS17-2
• Accession: NZ_CP061677
• Organism: Acinetobacter seifertii strain AS17

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: IC768_RS20875
• Protein ID: WP_000312250.1
• Coordinates: 64652..65011 (+)
• Length: 120 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: IC768_RS20880
• Protein ID: WP_001096429.1
• Coordinates: 65011..65310 (+)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IC768_RS20840	59976..60290	-	315	WP_032011240.1	hypothetical protein	-
IC768_RS20845	61156..61491	-	336	WP_000654056.1	hypothetical protein	-
IC768_RS20850	61543..62301	-	759	WP_001053130.1	hypothetical protein	-
IC768_RS20855	62368..62751	-	384	WP_032018724.1	hypothetical protein	-
IC768_RS20860	62889..63587	-	699	WP_000873188.1	hypothetical protein	-
IC768_RS20865	63606..63836	-	231	WP_045124240.1	hypothetical protein	-
IC768_RS20870	63885..64451	-	567	WP_031948745.1	hypothetical protein	-
IC768_RS20875	64652..65011	+	360	WP_000312250.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
IC768_RS20880	65011..65310	+	300	WP_001096429.1	helix-turn-helix transcriptional regulator	Antitoxin
IC768_RS20885	65846..66382	-	537	WP_032010581.1	hypothetical protein	-
IC768_RS20890	66432..66986	-	555	WP_000790086.1	hypothetical protein	-
IC768_RS20895	67067..67576	-	510	WP_032018705.1	hypothetical protein	-
IC768_RS20900	67616..68245	-	630	WP_002081923.1	hypothetical protein	-
IC768_RS20905	68262..68441	-	180	WP_002081921.1	hypothetical protein	-
IC768_RS20910	68417..68989	-	573	WP_032011345.1	hypothetical protein	-
IC768_RS20915	68994..69251	-	258	WP_000834292.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	blaOXA-82	-	1..74124	74124

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (14-109)

Antitoxin

• HigA2-like, helix-turn-helix domain (12-88)

Sequences

Toxin

Protein

• Length: 120 a.a.
• Molecular weight: 13751.62 Da
• Isoelectric Point: 4.8212

>T172784 WP_000312250.1 NZ_CP061677:64652-65011 [Acinetobacter seifertii]
MAWDVETTELFDSWLAEQDENAQDKILASLLVLSELGPNLGRPHVDTIKESKYPNMKEIRVQVKGHPIRGFFAFDPERKA
IVLCAGDKKGLNEKAFYKEMIKIADEQYEQYLRDNYGDK

Nucleotide

• Length: 360 bp

>T172784 NZ_CP079924:3603295-3603573 [Escherichia sp. TC-EC600-tetX4]
ATGATTCAAAGGGATATTGAATACTCGGGACAATATTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATACTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 11012.93 Da
• Isoelectric Point: 10.4856

>AT172784 WP_001096429.1 NZ_CP061677:65011-65310 [Acinetobacter seifertii]
MAKLNDIISKRSPESQQRIKKMANQMLLDINLSNLREELKISQAEIAKNLGVSQAAISKREKLGKDLKISSLKEYVEAMG
GKVRLDVELPNGKHVAISI

Nucleotide

• Length: 300 bp

>AT172784 NZ_CP079924:3603032-3603292 [Escherichia sp. TC-EC600-tetX4]
ATGGCTGCTAACGCGTTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCAGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCATTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA