Detailed information of TA system TA172783

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 54236..54887
• Replicon: plasmid pAS17-1
• Accession: NZ_CP061676
• Organism: Acinetobacter seifertii strain AS17

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A7H2WXC0
• Locus tag: IC768_RS20180
• Protein ID: WP_006581604.1
• Coordinates: 54531..54887 (-)
• Length: 119 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: IC768_RS20175
• Protein ID: WP_001140619.1
• Coordinates: 54236..54538 (-)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IC768_RS20140	49272..49460	-	189	WP_000033474.1	type II toxin-antitoxin system RelE/ParE family toxin	-
IC768_RS20145	49737..50057	-	321	WP_002123990.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
IC768_RS20150	50243..50556	+	314	Protein_55	AAA family ATPase	-
IC768_RS20155	50636..51795	+	1160	WP_085945698.1	IS3 family transposase	-
IC768_RS20160	52172..53331	+	1160	WP_085945698.1	IS3 family transposase	-
IC768_RS20165	53706..53924	+	219	WP_029747731.1	hypothetical protein	-
IC768_RS20170	54034..54243	-	210	WP_000069471.1	hypothetical protein	-
IC768_RS20175	54236..54538	-	303	WP_001140619.1	XRE family transcriptional regulator	Antitoxin
IC768_RS20180	54531..54887	-	357	WP_006581604.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
IC768_RS20185	55697..57151	+	1455	WP_062032131.1	SulP family inorganic anion transporter	-
IC768_RS20190	57365..57727	-	363	Protein_63	DNA-binding protein	-
IC768_RS20195	57761..57937	-	177	WP_006581600.1	hypothetical protein	-
IC768_RS20200	58222..59430	+	1209	WP_004832869.1	IS256-like element ISAba26 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..107398	107398
	-	inside	IScluster/Tn	-	-	50636..59430	8794

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-106)

Antitoxin

• HigA2-like, helix-turn-helix domain (13-89)

Sequences

Toxin

Protein

• Length: 119 a.a.
• Molecular weight: 13462.38 Da
• Isoelectric Point: 7.1642

>T172783 WP_006581604.1 NZ_CP061676:c54887-54531 [Acinetobacter seifertii]
MWTVITTDLFNEWLAQQDQSTQEKVLAALVVLQQQGPSLGRPLVDTVYDSKFTNMKDLRVQHRGKSLRAFFAFDPLRQAI
VLCIGDKGGKKRFYKEMLDIADQQYEIHLSTLGDQSNG

Nucleotide

• Length: 357 bp

>T172783 NZ_CP079924:c3597233-3596835 [Escherichia sp. TC-EC600-tetX4]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCCTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGAGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11206.01 Da
• Isoelectric Point: 6.7282

>AT172783 WP_001140619.1 NZ_CP061676:c54538-54236 [Acinetobacter seifertii]
MAKTLQELLANRSPESQARIQQMADELLLETQLHLIREELEISQKELAATLGIKQPSLSAIENRGHDLKISTMKKYVEAM
GGKLRIDVELPTGKHIGFNV

Nucleotide

• Length: 303 bp

>AT172783 NZ_CP079924:c3597529-3597236 [Escherichia sp. TC-EC600-tetX4]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGCGCT
GAACAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Structures

Toxin

• AlphaFold DB: A0A7H2WXC0