Detailed information of TA system TA172777

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 46932..47590
• Replicon: plasmid pAS31-2
• Accession: NZ_CP061662
• Organism: Acinetobacter seifertii strain AS31

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: IC773_RS20720
• Protein ID: WP_000312250.1
• Coordinates: 47231..47590 (-)
• Length: 120 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: IC773_RS20715
• Protein ID: WP_001096429.1
• Coordinates: 46932..47231 (-)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IC773_RS20680	42992..43249	+	258	WP_000834292.1	hypothetical protein	-
IC773_RS20685	43254..43826	+	573	WP_032011345.1	hypothetical protein	-
IC773_RS20690	43802..43981	+	180	WP_002081921.1	hypothetical protein	-
IC773_RS20695	43998..44627	+	630	WP_002081923.1	hypothetical protein	-
IC773_RS20700	44667..45175	+	509	Protein_58	hypothetical protein	-
IC773_RS20705	45256..45810	+	555	WP_000790086.1	hypothetical protein	-
IC773_RS20710	45860..46396	+	537	WP_032010581.1	hypothetical protein	-
IC773_RS20715	46932..47231	-	300	WP_001096429.1	helix-turn-helix transcriptional regulator	Antitoxin
IC773_RS20720	47231..47590	-	360	WP_000312250.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
IC773_RS20725	47791..48357	+	567	WP_031948745.1	hypothetical protein	-
IC773_RS20730	48406..48636	+	231	WP_045124240.1	hypothetical protein	-
IC773_RS20735	48655..49353	+	699	WP_000873188.1	hypothetical protein	-
IC773_RS20740	49491..49874	+	384	WP_032018724.1	hypothetical protein	-
IC773_RS20745	49941..50699	+	759	WP_001053130.1	hypothetical protein	-
IC773_RS20750	50751..51086	+	336	WP_000654056.1	hypothetical protein	-
IC773_RS20755	51952..52266	+	315	WP_032011240.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	blaOXA-82	-	1..75214	75214

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (14-109)

Antitoxin

• HigA2-like, helix-turn-helix domain (12-88)

Sequences

Toxin

Protein

• Length: 120 a.a.
• Molecular weight: 13751.62 Da
• Isoelectric Point: 4.8212

>T172777 WP_000312250.1 NZ_CP061662:c47590-47231 [Acinetobacter seifertii]
MAWDVETTELFDSWLAEQDENAQDKILASLLVLSELGPNLGRPHVDTIKESKYPNMKEIRVQVKGHPIRGFFAFDPERKA
IVLCAGDKKGLNEKAFYKEMIKIADEQYEQYLRDNYGDK

Nucleotide

• Length: 360 bp

>T172777 NZ_CP079924:2233510-2233797 [Escherichia sp. TC-EC600-tetX4]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 11012.93 Da
• Isoelectric Point: 10.4856

>AT172777 WP_001096429.1 NZ_CP061662:c47231-46932 [Acinetobacter seifertii]
MAKLNDIISKRSPESQQRIKKMANQMLLDINLSNLREELKISQAEIAKNLGVSQAAISKREKLGKDLKISSLKEYVEAMG
GKVRLDVELPNGKHVAISI

Nucleotide

• Length: 300 bp

>AT172777 NZ_CP079924:2233271-2233510 [Escherichia sp. TC-EC600-tetX4]
ATGGGTAGCATTAACCTGCGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGTGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGGCTTCGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA