Detailed information of TA system TA172776

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 64129..64780
• Replicon: plasmid pAS31-1
• Accession: NZ_CP061661
• Organism: Acinetobacter seifertii strain AS31

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A7H2WXC0
• Locus tag: IC773_RS20200
• Protein ID: WP_006581604.1
• Coordinates: 64424..64780 (-)
• Length: 119 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: IC773_RS20195
• Protein ID: WP_001140619.1
• Coordinates: 64129..64431 (-)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IC773_RS20150	59548..59845	+	298	Protein_62	hypothetical protein	-
IC773_RS20155	59796..60050	+	255	WP_000236586.1	hypothetical protein	-
IC773_RS20160	60129..60418	-	290	Protein_64	helix-turn-helix domain-containing protein	-
IC773_RS20165	60405..60593	-	189	WP_000033474.1	type II toxin-antitoxin system RelE/ParE family toxin	-
IC773_RS20170	60870..61190	-	321	WP_002123990.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
IC773_RS20175	61376..61689	+	314	Protein_67	AAA family ATPase	-
IC773_RS20180	61769..62928	+	1160	WP_085945698.1	IS3 family transposase	-
IC773_RS20185	63599..63817	+	219	WP_029747731.1	hypothetical protein	-
IC773_RS20190	63927..64136	-	210	WP_000069471.1	hypothetical protein	-
IC773_RS20195	64129..64431	-	303	WP_001140619.1	XRE family transcriptional regulator	Antitoxin
IC773_RS20200	64424..64780	-	357	WP_006581604.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
IC773_RS20205	65590..67044	+	1455	WP_062032131.1	SulP family inorganic anion transporter	-
IC773_RS20210	67258..67620	-	363	Protein_74	DNA-binding protein	-
IC773_RS20215	67654..67830	-	177	WP_006581600.1	hypothetical protein	-
IC773_RS20220	68115..69323	+	1209	WP_004832869.1	IS256-like element ISAba26 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..105815	105815
	-	flank	IS/Tn	-	-	68115..69323	1208

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-106)

Antitoxin

• HigA2-like, helix-turn-helix domain (13-89)

Sequences

Toxin

Protein

• Length: 119 a.a.
• Molecular weight: 13462.38 Da
• Isoelectric Point: 7.1642

>T172776 WP_006581604.1 NZ_CP061661:c64780-64424 [Acinetobacter seifertii]
MWTVITTDLFNEWLAQQDQSTQEKVLAALVVLQQQGPSLGRPLVDTVYDSKFTNMKDLRVQHRGKSLRAFFAFDPLRQAI
VLCIGDKGGKKRFYKEMLDIADQQYEIHLSTLGDQSNG

Nucleotide

• Length: 357 bp

>T172776 NZ_CP079924:1955936-1956039 [Escherichia sp. TC-EC600-tetX4]
GGCAAGGCAACTAAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTGTTCGGTCTCTTTTT

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11206.01 Da
• Isoelectric Point: 6.7282

>AT172776 WP_001140619.1 NZ_CP061661:c64431-64129 [Acinetobacter seifertii]
MAKTLQELLANRSPESQARIQQMADELLLETQLHLIREELEISQKELAATLGIKQPSLSAIENRGHDLKISTMKKYVEAM
GGKLRIDVELPTGKHIGFNV

Nucleotide

• Length: 303 bp

>AT172776 NZ_CP079924:c1956077-1955806 [Escherichia sp. TC-EC600-tetX4]
AAAGTCAGCGAAGGAAATGCTTCTGGCTTTTAACAGATAAAAAGAGACCGAACACGATTCCTGTATTCGGTCCAGGGAAA
TGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGCATTAATGCAGGCTTAGTTGCCTTGCCCTTTAAGAATAGATGACG
ACGCCAGGTTTTCCAGTTTGCGTGCAAAATGGTCAATAAAAAGCGTGGTGGTCATCAGCTGAAATGTTAAAAACCGCCCG
TTCTGGTGAAAGAACTGAGGCGGTTTTTTTAT

Structures

Toxin

• AlphaFold DB: A0A7H2WXC0