Detailed information of TA system TA172773

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/Tad-HTH_37
• Location: 87496..87958
• Replicon: plasmid pAS39-1
• Accession: NZ_CP061647
• Organism: Acinetobacter seifertii strain AS39

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A3R9PS35
• Locus tag: IC776_RS19005
• Protein ID: WP_000033474.1
• Coordinates: 87496..87684 (+)
• Length: 63 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: IC776_RS19010
• Protein ID: WP_001258992.1
• Coordinates: 87671..87958 (+)
• Length: 96 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IC776_RS18965	82825..83343	+	519	WP_000447197.1	sel1 repeat family protein	-
IC776_RS18970	83902..84258	+	357	WP_000269901.1	type II toxin-antitoxin system RelE/ParE family toxin	-
IC776_RS18975	84251..84553	+	303	WP_001140619.1	XRE family transcriptional regulator	-
IC776_RS18980	84546..84755	+	210	WP_000069471.1	hypothetical protein	-
IC776_RS18985	84863..85090	-	228	WP_000286812.1	hypothetical protein	-
IC776_RS18990	85849..86394	-	546	Protein_99	IS5-like element ISAba13 family transposase	-
IC776_RS18995	86322..86711	-	390	WP_002072923.1	AAA family ATPase	-
IC776_RS19000	86899..87219	+	321	WP_000088946.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
IC776_RS19005	87496..87684	+	189	WP_000033474.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
IC776_RS19010	87671..87958	+	288	WP_001258992.1	helix-turn-helix transcriptional regulator	Antitoxin
IC776_RS19015	88037..88291	-	255	WP_000236586.1	hypothetical protein	-
IC776_RS19020	88242..88466	-	225	WP_004882762.1	hypothetical protein	-
IC776_RS19025	88490..89047	-	558	Protein_106	hypothetical protein	-
IC776_RS19030	89087..90399	-	1313	Protein_107	terminase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..90902	90902
	-	inside	IScluster/Tn	-	-	80145..86274	6129

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (2-55)

Antitoxin

• Cro/C1-type helix-turn-helix domain (39-90)

Sequences

Toxin

Protein

• Length: 63 a.a.
• Molecular weight: 7137.56 Da
• Isoelectric Point: 10.9048

>T172773 WP_000033474.1 NZ_CP061647:87496-87684 [Acinetobacter seifertii]
MSKGLFELRLKSQEGIARVMYCTLVGKRIVMLHSFVKKTQKTPKQDLNLALDRMKEVKNANT

Nucleotide

• Length: 189 bp

>T172773 NZ_CP079924:1790206-1790457 [Escherichia sp. TC-EC600-tetX4]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGCTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATCATTGAGTGA

Antitoxin

Protein

• Length: 96 a.a.
• Molecular weight: 10563.32 Da
• Isoelectric Point: 9.7308

>AT172773 WP_001258992.1 NZ_CP061647:87671-87958 [Acinetobacter seifertii]
MRTHEEMKALALSRSAVRTEYERIEREEMPLLDMVLTALREAGLSQAQIAERMGTNVPAVSRLEKALITGKPSPSIATLQ
KYAAAIGKHVEVRFV

Nucleotide

• Length: 288 bp

>AT172773 NZ_CP079924:1790454-1790708 [Escherichia sp. TC-EC600-tetX4]
GTGAAACTAATCTGGTCTGAGGAATCATGGGACGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Structures

Toxin

• AlphaFold DB: A0A3R9PS35