Detailed information of TA system TA172772

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 83902..84553
• Replicon: plasmid pAS39-1
• Accession: NZ_CP061647
• Organism: Acinetobacter seifertii strain AS39

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A0E2G754
• Locus tag: IC776_RS18970
• Protein ID: WP_000269901.1
• Coordinates: 83902..84258 (+)
• Length: 119 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: IC776_RS18975
• Protein ID: WP_001140619.1
• Coordinates: 84251..84553 (+)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IC776_RS18945	79075..79176	+	102	Protein_90	DNA-binding protein	-
IC776_RS18950	79374..79550	+	177	WP_191011570.1	hypothetical protein	-
IC776_RS18955	80145..81470	-	1326	WP_001280604.1	ISNCY family transposase	-
IC776_RS18960	81634..82146	-	513	WP_001089571.1	SMI1/KNR4 family protein	-
IC776_RS18965	82825..83343	+	519	WP_000447197.1	sel1 repeat family protein	-
IC776_RS18970	83902..84258	+	357	WP_000269901.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
IC776_RS18975	84251..84553	+	303	WP_001140619.1	XRE family transcriptional regulator	Antitoxin
IC776_RS18980	84546..84755	+	210	WP_000069471.1	hypothetical protein	-
IC776_RS18985	84863..85090	-	228	WP_000286812.1	hypothetical protein	-
IC776_RS18990	85849..86394	-	546	Protein_99	IS5-like element ISAba13 family transposase	-
IC776_RS18995	86322..86711	-	390	WP_002072923.1	AAA family ATPase	-
IC776_RS19000	86899..87219	+	321	WP_000088946.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
IC776_RS19005	87496..87684	+	189	WP_000033474.1	type II toxin-antitoxin system RelE/ParE family toxin	-
IC776_RS19010	87671..87958	+	288	WP_001258992.1	helix-turn-helix transcriptional regulator	-
IC776_RS19015	88037..88291	-	255	WP_000236586.1	hypothetical protein	-
IC776_RS19020	88242..88466	-	225	WP_004882762.1	hypothetical protein	-
IC776_RS19025	88490..89047	-	558	Protein_106	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..90902	90902
	-	inside	IScluster/Tn	-	-	80145..86274	6129

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-106)

Antitoxin

• HigA2-like, helix-turn-helix domain (13-89)

Sequences

Toxin

Protein

• Length: 119 a.a.
• Molecular weight: 13487.44 Da
• Isoelectric Point: 6.4631

>T172772 WP_000269901.1 NZ_CP061647:83902-84258 [Acinetobacter seifertii]
MWTVITTDLFNEWLAQQDESTQEKVLAALVVLQQQGPSLGRPLVDTVYDSKFTNMKELRVQHRGKPLRAFFAFDPLRQAI
VLCIGDKGGKKRFYKEMLDIADQQYEIHLSTLGDQSNG

Nucleotide

• Length: 357 bp

>T172772 NZ_CP079924:c1119850-1118777 [Escherichia sp. TC-EC600-tetX4]
ATGACAATCAGGAGTTACAAAAACTTAAATCTGGTCAGGGCAAATATCGAGACTGAATCCAGACAATTCATTGAAAATAA
AAACTATTCAATCCAATCAATTGGTCCTATGCCAGGGTCAAGGGCTGGGCTTCGGGTCGTATTTACCAGACCAGGGGTTA
ACTTGGCAACTGTGGACATTTTTTATAACGGGGACGGTTCGACTACAATTCAATATCTCACTGGAGCCAATCGTTCTCTG
GGCCAAGAGTTAGCGGATCATCTTTTTGAAACCATCAATCCTGCTGAATTTGAGCAGGTAAATATGGTACTGCAAGGATT
TGTAGAGACAAGCGTTCTACCTGTACTTGAGCTATCAGCAGATGAATCGCATATAGAGTTCAGAGAACACTCTCGTAACG
CTCATACCGTAGTGTGGAAAATTATTTCCACCAGCTATCAGGACGAATTGACTGTGAGCCTGCATATCACAACAGGTAAG
CTCCAGATTCAGGGCCGACCGCTGTCATGTTACAGAGTTTTCACGTTTAACTTGGCAGCCCTGCTTGATTTACAGGGTTT
GGAGAAAGTGCTAATCCGCCAGGAGGATGGTAAAGCTAATATTGTTCAACAGGAGGTTGCCCGCACTTACTTGCAGACTG
TAATGGCCGATGCTTACCCGCATCTCCACGTGACTGCCGAAAAATTGCTCGTTTCAGGGCTATGTGTTAAACTCGCCGCC
CCTGATTTGCCTGACTACTGTATGTTACTTTATCCTGAACTACGCACCATTGAAGGTGTCTTAAAAAGTAAGATGAGTGG
GTTAGGCATGCCAGTACAGCAGCCGGCAGGTTTTGGAACTTACTTTGATAAACCTGCTGCTCATTACATTCTGAAACCGC
AATTTGCAGCTACTCTTAGACCGGAACAGATTAACATCATCAGCACAGCCTATACTTTTTTTAATGTGGAACGTCATTCT
CTGTTCCACATGGAAACTGTGGTCGATGCCAGCCGTATGATTTCTGATATGGCCCGGTTGATGGGTAAAGCCACTAGAGC
GTGGGGAATAATCAAGGACTTATATATTGTTTGA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11206.01 Da
• Isoelectric Point: 6.7282

>AT172772 WP_001140619.1 NZ_CP061647:84251-84553 [Acinetobacter seifertii]
MAKTLQELLANRSPESQARIQQMADELLLETQLHLIREELEISQKELAATLGIKQPSLSAIENRGHDLKISTMKKYVEAM
GGKLRIDVELPTGKHIGFNV

Nucleotide

• Length: 303 bp

>AT172772 NZ_CP079924:c1118784-1118413 [Escherichia sp. TC-EC600-tetX4]
TTGTTTGAAATCACCGGAATTAACGTCTCAGGGGCTCTAAAAGCTGTCGTTATGGCGACCGGCTTCGAGAACCCTTTGTC
CTCAGTTAATGAAATTGAGACGAAACTTTCTGCCCTACTGGGTAGTGAGACCACTGGTGAAATCCTTTTTGACCTCTTGT
GCGCTAACGGTCCAGAGTGGAACCGCTTCGTTACGCTGGAAATGAAATATGGCCGGATCATGCTGGATACGGCCAAAATT
ATTGATGAGCAGGACGTTCCGACACATATCCTGAGCAAACTGACTTTTACTTTACGTAATCATCCTGAGTACCTAGAAGC
CTCTGTTTTGTCTCCCGATGATGTACGCCAAGTCCTGTCAATGGATTTTTGA

Structures

Toxin

• AlphaFold DB: A0A0E2G754