Detailed information of TA system TA172770

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 47496..48147
• Replicon: plasmid pAS40-2
• Accession: NZ_CP061642
• Organism: Acinetobacter seifertii strain AS40

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A7H2WXC0
• Locus tag: IC777_RS20860
• Protein ID: WP_006581604.1
• Coordinates: 47791..48147 (-)
• Length: 119 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: IC777_RS20855
• Protein ID: WP_001140619.1
• Coordinates: 47496..47798 (-)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IC777_RS20810	42915..43212	+	298	Protein_45	hypothetical protein	-
IC777_RS20815	43163..43417	+	255	WP_000236586.1	hypothetical protein	-
IC777_RS20820	43496..43785	-	290	Protein_47	helix-turn-helix domain-containing protein	-
IC777_RS20825	43772..43960	-	189	WP_000033474.1	type II toxin-antitoxin system RelE/ParE family toxin	-
IC777_RS20830	44237..44557	-	321	WP_002123990.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
IC777_RS20835	44743..45056	+	314	Protein_50	AAA family ATPase	-
IC777_RS20840	45136..46295	+	1160	WP_085945698.1	IS3 family transposase	-
IC777_RS20845	46966..47184	+	219	WP_029747731.1	hypothetical protein	-
IC777_RS20850	47294..47503	-	210	WP_000069471.1	hypothetical protein	-
IC777_RS20855	47496..47798	-	303	WP_001140619.1	XRE family transcriptional regulator	Antitoxin
IC777_RS20860	47791..48147	-	357	WP_006581604.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
IC777_RS20865	48957..50411	+	1455	WP_062032131.1	SulP family inorganic anion transporter	-
IC777_RS20870	50625..50987	-	363	Protein_57	DNA-binding protein	-
IC777_RS20875	51021..51197	-	177	WP_006581600.1	hypothetical protein	-
IC777_RS20880	51482..52690	+	1209	WP_004832869.1	IS256-like element ISAba26 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..106923	106923
	-	flank	IS/Tn	-	-	51482..52690	1208

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-106)

Antitoxin

• HigA2-like, helix-turn-helix domain (13-89)

Sequences

Toxin

Protein

• Length: 119 a.a.
• Molecular weight: 13462.38 Da
• Isoelectric Point: 7.1642

>T172770 WP_006581604.1 NZ_CP061642:c48147-47791 [Acinetobacter seifertii]
MWTVITTDLFNEWLAQQDQSTQEKVLAALVVLQQQGPSLGRPLVDTVYDSKFTNMKDLRVQHRGKSLRAFFAFDPLRQAI
VLCIGDKGGKKRFYKEMLDIADQQYEIHLSTLGDQSNG

Nucleotide

• Length: 357 bp

>T172770 NZ_CP079924:974677-975012 [Escherichia sp. TC-EC600-tetX4]
ATGGTAAGCCGATACGTACCCGATATGGGCGATCTGATTTGGGTTGATTTTGACCCGACAAAAGGTAGCGAGCAAGCTGG
ACATCGTCCAGCTGTTGTCCTGAGTCCTTTCATGTACAACAACAAAACAGGTATGTGTCTGTGTGTTCCTTGTACAACGC
AATCAAAAGGATATCCGTTCGAAGTTGTTTTATCCGGTCAGGAACGTGATGGCGTAGCGTTAGCTGATCAGGTAAAAAGT
ATCGCCTGGCGGGCAAGAGGAGCAACGAAGAAAGGAACAGTTGCCCCAGAGGAATTACAACTCATTAAAGCCAAAATTAA
CGTACTGATTGGGTAG

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11206.01 Da
• Isoelectric Point: 6.7282

>AT172770 WP_001140619.1 NZ_CP061642:c47798-47496 [Acinetobacter seifertii]
MAKTLQELLANRSPESQARIQQMADELLLETQLHLIREELEISQKELAATLGIKQPSLSAIENRGHDLKISTMKKYVEAM
GGKLRIDVELPTGKHIGFNV

Nucleotide

• Length: 303 bp

>AT172770 NZ_CP079924:974429-974677 [Escherichia sp. TC-EC600-tetX4]
ATGATCCACAGTAGCGTAAAGCGTTGGGGAAATTCACCGGCGGTGCGGATCCCGGCTACGTTAATGCAGGCGCTCAATCT
GAATATTGATGATGAAGTGAAGATTGACCTGGTGGATGGCAAATTAATTATTGAGCCAGTGCGTAAAGAGCCCGTATTTA
CGCTTGCTGAACTGGTCAACGACATCACGCCGGAAAACCTCCACGAGAATATCGACTGGGGAGAGCCGAAAGATAAGGAA
GTCTGGTAA

Structures

Toxin

• AlphaFold DB: A0A7H2WXC0