Detailed information of TA system TA172768

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 41445..42103
• Replicon: plasmid pAS41-3
• Accession: NZ_CP061637
• Organism: Acinetobacter seifertii strain AS41

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: IC778_RS21060
• Protein ID: WP_000312250.1
• Coordinates: 41445..41804 (+)
• Length: 120 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: IC778_RS21065
• Protein ID: WP_001096429.1
• Coordinates: 41804..42103 (+)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IC778_RS21025	36769..37083	-	315	WP_032011240.1	hypothetical protein	-
IC778_RS21030	37949..38284	-	336	WP_000654056.1	hypothetical protein	-
IC778_RS21035	38336..39094	-	759	WP_001053130.1	hypothetical protein	-
IC778_RS21040	39161..39544	-	384	WP_032018724.1	hypothetical protein	-
IC778_RS21045	39682..40380	-	699	WP_000873188.1	hypothetical protein	-
IC778_RS21050	40399..40629	-	231	WP_045124240.1	hypothetical protein	-
IC778_RS21055	40678..41244	-	567	WP_031948745.1	hypothetical protein	-
IC778_RS21060	41445..41804	+	360	WP_000312250.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
IC778_RS21065	41804..42103	+	300	WP_001096429.1	helix-turn-helix transcriptional regulator	Antitoxin
IC778_RS21070	42639..43175	-	537	WP_032010581.1	hypothetical protein	-
IC778_RS21075	43225..43779	-	555	WP_000790086.1	hypothetical protein	-
IC778_RS21080	43860..44369	-	510	WP_032018705.1	hypothetical protein	-
IC778_RS21085	44409..45038	-	630	WP_002081923.1	hypothetical protein	-
IC778_RS21090	45055..45234	-	180	WP_002081921.1	hypothetical protein	-
IC778_RS21095	45210..45782	-	573	WP_032011345.1	hypothetical protein	-
IC778_RS21100	45787..46044	-	258	WP_000834292.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	blaOXA-82	-	1..74127	74127

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (14-109)

Antitoxin

• HigA2-like, helix-turn-helix domain (12-88)

Sequences

Toxin

Protein

• Length: 120 a.a.
• Molecular weight: 13751.62 Da
• Isoelectric Point: 4.8212

>T172768 WP_000312250.1 NZ_CP061637:41445-41804 [Acinetobacter seifertii]
MAWDVETTELFDSWLAEQDENAQDKILASLLVLSELGPNLGRPHVDTIKESKYPNMKEIRVQVKGHPIRGFFAFDPERKA
IVLCAGDKKGLNEKAFYKEMIKIADEQYEQYLRDNYGDK

Nucleotide

• Length: 360 bp

>T172768 NZ_CP079924:717224-717520 [Escherichia sp. TC-EC600-tetX4]
ATGGAAAAACGCACACCACATACACGTTTGAGTCAGGTTAAAAAACTTGTCAATGCCGGGCAAGTTCGTACAACACGTAG
TGCCCTGTTAAATGCAGATGAGTTAGGTTTGGATTTTGATGGTATGTGTAATGTTATCATTGGATTATCAGAGAGCGACT
TTTATAAAAGCATGACCACCTACTCTGATCATACTATCTGGCAGGATGTTTACAGACCCAGGCTTGTTACAGGCCAGGTT
TATCTTAAAATTACGGTAATTCATGACGTACTGATCGTCTCGTTTAAGGAGAAGTAA

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 11012.93 Da
• Isoelectric Point: 10.4856

>AT172768 WP_001096429.1 NZ_CP061637:41804-42103 [Acinetobacter seifertii]
MAKLNDIISKRSPESQQRIKKMANQMLLDINLSNLREELKISQAEIAKNLGVSQAAISKREKLGKDLKISSLKEYVEAMG
GKVRLDVELPNGKHVAISI

Nucleotide

• Length: 300 bp

>AT172768 NZ_CP079924:717522-717917 [Escherichia sp. TC-EC600-tetX4]
ATGAAATGTCCGGTTTGCCACCAGGGAGAAATGGTTTCTGGCATTAAAGATATTCCATACACCTTCCGTGGACGAAAAAC
AGTATTGAAAGGTATCCACGGTTTATATTGTGTCCATTGCGAAGAGAGCATCATGAATAAAGAAGAGTCAGATGCTTTCA
TGGCGCAAGTAAAGGCATTTCGGGCTTCGGTGAATGCCGAAACAGTGGCACCTGAATTTATAGTGAAGGTTCGAAAAAAG
CTCTCTCTTACCCAAAAAGAGGCAAGCGAAATTTTTGGGGGAGGTGTAAATGCGTTTTCGCGTTACGAAAAAGGCAATGC
CCAACCTCATCCTTCCACAATCAAACTTTTACGTGTTCTGGATAAGCATCCAGAACTATTGAATGAAATCCGTTAA