Detailed information of TA system TA172767

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 17925..18576
• Replicon: plasmid pAS41-2
• Accession: NZ_CP061636
• Organism: Acinetobacter seifertii strain AS41

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A7H2WXC0
• Locus tag: IC778_RS20340
• Protein ID: WP_006581604.1
• Coordinates: 17925..18281 (+)
• Length: 119 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: IC778_RS20345
• Protein ID: WP_001140619.1
• Coordinates: 18274..18576 (+)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IC778_RS20320	13382..14590	-	1209	WP_004832869.1	IS256-like element ISAba26 family transposase	-
IC778_RS20325	14875..15051	+	177	WP_006581600.1	hypothetical protein	-
IC778_RS20330	15085..15447	+	363	Protein_17	DNA-binding protein	-
IC778_RS20335	15661..17115	-	1455	WP_062032131.1	SulP family inorganic anion transporter	-
IC778_RS20340	17925..18281	+	357	WP_006581604.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
IC778_RS20345	18274..18576	+	303	WP_001140619.1	XRE family transcriptional regulator	Antitoxin
IC778_RS20350	18569..18778	+	210	WP_000069471.1	hypothetical protein	-
IC778_RS20355	18888..19106	-	219	WP_029747731.1	hypothetical protein	-
IC778_RS20365	21016..21329	-	314	Protein_24	AAA family ATPase	-
IC778_RS20370	21515..21835	+	321	WP_002123990.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
IC778_RS20375	22112..22300	+	189	WP_000033474.1	type II toxin-antitoxin system RelE/ParE family toxin	-
IC778_RS20380	22287..22576	+	290	Protein_27	helix-turn-helix domain-containing protein	-
IC778_RS20385	22655..22909	-	255	WP_000236586.1	hypothetical protein	-
IC778_RS20390	22860..23157	-	298	Protein_29	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..105816	105816
	-	flank	IS/Tn	-	-	13382..14590	1208

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-106)

Antitoxin

• HigA2-like, helix-turn-helix domain (13-89)

Sequences

Toxin

Protein

• Length: 119 a.a.
• Molecular weight: 13462.38 Da
• Isoelectric Point: 7.1642

>T172767 WP_006581604.1 NZ_CP061636:17925-18281 [Acinetobacter seifertii]
MWTVITTDLFNEWLAQQDQSTQEKVLAALVVLQQQGPSLGRPLVDTVYDSKFTNMKDLRVQHRGKSLRAFFAFDPLRQAI
VLCIGDKGGKKRFYKEMLDIADQQYEIHLSTLGDQSNG

Nucleotide

• Length: 357 bp

>T172767 NZ_CP079924:651314-651628 [Escherichia sp. TC-EC600-tetX4]
ATGCACCTGATAACTCAAAAAGCATTGAAAGATGCTGCGGAAAAATACCCGCAACATAAAACGGAGTTGGTGGCTCTGGG
GAACACGATTGCTAAGGGATATTTCAAAAAACCTGAGTCATTAAAAGCAGTATTCCCATCTCTGGATAACTTCAAATATC
TGGATAAGCATTATGTTTTCAATGTTGGGGGCAATGAATTACGTGTTGTAGCAATGGTCTTTTTTGAATCGCAAAAGTGC
TACATACGTGAAGTTATGACGCATAAAGAATACGATTTCTTTACCGCTGTTCATCGTACTAAGGGGAAAAAATGA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11206.01 Da
• Isoelectric Point: 6.7282

>AT172767 WP_001140619.1 NZ_CP061636:18274-18576 [Acinetobacter seifertii]
MAKTLQELLANRSPESQARIQQMADELLLETQLHLIREELEISQKELAATLGIKQPSLSAIENRGHDLKISTMKKYVEAM
GGKLRIDVELPTGKHIGFNV

Nucleotide

• Length: 303 bp

>AT172767 NZ_CP079924:651625-652041 [Escherichia sp. TC-EC600-tetX4]
ATGATTGCGATTGCCGACATCTTGCAAGCAGGAGAAAAGCTAACTGCTGTGGCACCTTTTCTGGCGGGTATTCAGAACGA
GGAACAATACACCCAGGCGCTGGAACTGGTAGATCATCTGCTGCTCAACGATCCTGAAAACCCCTTGCTGGATCTGGTGT
GTGCCAAAATAACCGCGTGGGAAGAATCAGCGCCCGAATTTGCGGAATTTAATGCCATGGCTCAAGCCATGCCTGGCGGT
ATAGCCGTGATTCGTACCCTTATGGATCAATATGGTTTAACCCTTTCCGATCTGCCGGAAATTGGCAGTAAATCTATGGT
GTCACGCGTTTTGAGCGGGAAGAGGAAATTAACGCTGGAACACGCTAAAAAATTGGCAACGCGATTCGGCATTTCTCCCG
CCTTGTTTATTGATTAA

Structures

Toxin

• AlphaFold DB: A0A7H2WXC0