Detailed information of TA system TA172764

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 64340..64991
• Replicon: plasmid pAS47-2
• Accession: NZ_CP061631
• Organism: Acinetobacter seifertii strain AS47

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A7H2WXC0
• Locus tag: IC780_RS20925
• Protein ID: WP_006581604.1
• Coordinates: 64635..64991 (-)
• Length: 119 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: IC780_RS20920
• Protein ID: WP_001140619.1
• Coordinates: 64340..64642 (-)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IC780_RS20875	59759..60056	+	298	Protein_62	hypothetical protein	-
IC780_RS20880	60007..60261	+	255	WP_000236586.1	hypothetical protein	-
IC780_RS20885	60340..60629	-	290	Protein_64	helix-turn-helix domain-containing protein	-
IC780_RS20890	60616..60804	-	189	WP_000033474.1	type II toxin-antitoxin system RelE/ParE family toxin	-
IC780_RS20895	61081..61401	-	321	WP_002123990.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
IC780_RS20900	61587..61900	+	314	Protein_67	AAA family ATPase	-
IC780_RS20905	61980..63139	+	1160	WP_085945698.1	IS3 family transposase	-
IC780_RS20910	63810..64028	+	219	WP_029747731.1	hypothetical protein	-
IC780_RS20915	64138..64347	-	210	WP_000069471.1	hypothetical protein	-
IC780_RS20920	64340..64642	-	303	WP_001140619.1	XRE family transcriptional regulator	Antitoxin
IC780_RS20925	64635..64991	-	357	WP_006581604.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
IC780_RS20930	65801..67255	+	1455	WP_062032131.1	SulP family inorganic anion transporter	-
IC780_RS20935	67469..67831	-	363	Protein_74	DNA-binding protein	-
IC780_RS20940	67865..68041	-	177	WP_006581600.1	hypothetical protein	-
IC780_RS20945	68326..69534	+	1209	WP_004832869.1	IS256-like element ISAba26 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..105811	105811
	-	flank	IS/Tn	-	-	68326..69534	1208

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-106)

Antitoxin

• HigA2-like, helix-turn-helix domain (13-89)

Sequences

Toxin

Protein

• Length: 119 a.a.
• Molecular weight: 13462.38 Da
• Isoelectric Point: 7.1642

>T172764 WP_006581604.1 NZ_CP061631:c64991-64635 [Acinetobacter seifertii]
MWTVITTDLFNEWLAQQDQSTQEKVLAALVVLQQQGPSLGRPLVDTVYDSKFTNMKDLRVQHRGKSLRAFFAFDPLRQAI
VLCIGDKGGKKRFYKEMLDIADQQYEIHLSTLGDQSNG

Nucleotide

• Length: 357 bp

>T172764 NZ_CP079923:c1579470-1579291 [Streptococcus pneumoniae]
ATGCCTATGACACAAAAAGAGATGGTTAAACTCCTTACGGCCCATGGTTGGATAAAGACTAGAGGCGGTAAAGGCTCCCA
TATTAAAATGGAAAAGCAAGGGGAAAGACCTATCACAATCCTTCATGGTAAGCTGAATAAGTACACTGAAAGAGGGATAA
GAAAGCAAGCTGGGTTGTAA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11206.01 Da
• Isoelectric Point: 6.7282

>AT172764 WP_001140619.1 NZ_CP061631:c64642-64340 [Acinetobacter seifertii]
MAKTLQELLANRSPESQARIQQMADELLLETQLHLIREELEISQKELAATLGIKQPSLSAIENRGHDLKISTMKKYVEAM
GGKLRIDVELPTGKHIGFNV

Nucleotide

• Length: 303 bp

>AT172764 NZ_CP079923:c1579254-1578802 [Streptococcus pneumoniae]
ATGTTAGTTACGTATCCAGCTTTATTTTATTATGACGACACAGACGGAACAGAAGCGACTTATTTTGTCCATTTCCCAGA
TTTTGAATACTCAGCTACACAAGGAGAGGGGATTTCTGAGGCTTTGGCTATGGGGTCGGAGTGGCTAGGGATAACTGTTG
CAGATTTGATAGAGAGTGATGGAGAGTTACCTCAGCCATCAGATATTAATAGCTTGTCTTTAATTGATAATGATCCTTTT
AAAGATGATGAAGATTTCGTGTCAACCTATGACCTTGATAAATCTTTTATTTCTATGGTATCTGTTGATGTATCAGAATA
CCTAGGAAGTCAGGAACCCATTAAAAAGACTTTGACTATACCAAAATGGGCAGATAAGTTGGGACGAGAAATGGGACTTA
ACTTTTCTCAGACTTTGACAGACGCTATTGCAGATAAGAAAGTTCAAGCCTAA

Structures

Toxin

• AlphaFold DB: A0A7H2WXC0