Detailed information of TA system TA172761

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 36981..37632
• Replicon: plasmid pAS48-1
• Accession: NZ_CP061627
• Organism: Acinetobacter seifertii strain AS48

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A0E2G754
• Locus tag: IC781_RS18735
• Protein ID: WP_000269901.1
• Coordinates: 37276..37632 (-)
• Length: 119 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: IC781_RS18730
• Protein ID: WP_001140619.1
• Coordinates: 36981..37283 (-)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IC781_RS18680	32486..33043	+	558	Protein_37	hypothetical protein	-
IC781_RS18685	33067..33291	+	225	WP_004882762.1	hypothetical protein	-
IC781_RS18690	33242..33496	+	255	WP_000236586.1	hypothetical protein	-
IC781_RS18695	33575..33862	-	288	WP_001258992.1	helix-turn-helix transcriptional regulator	-
IC781_RS18700	33849..34037	-	189	WP_000033474.1	type II toxin-antitoxin system RelE/ParE family toxin	-
IC781_RS18705	34314..34634	-	321	WP_000088946.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
IC781_RS18710	34822..35211	+	390	WP_002072923.1	AAA family ATPase	-
IC781_RS18715	35139..35684	+	546	Protein_44	IS5-like element ISAba13 family transposase	-
IC781_RS18720	36444..36671	+	228	WP_000286812.1	hypothetical protein	-
IC781_RS18725	36779..36988	-	210	WP_000069471.1	hypothetical protein	-
IC781_RS18730	36981..37283	-	303	WP_001140619.1	XRE family transcriptional regulator	Antitoxin
IC781_RS18735	37276..37632	-	357	WP_000269901.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
IC781_RS18740	38214..38720	+	507	WP_191011569.1	sel1 repeat family protein	-
IC781_RS18745	38878..40203	+	1326	WP_016805832.1	ISNCY family transposase	-
IC781_RS18750	40797..40973	-	177	WP_191011570.1	hypothetical protein	-
IC781_RS18755	41171..41272	-	102	Protein_52	DNA-binding protein	-
IC781_RS18760	41264..41485	-	222	Protein_53	IS3 family transposase	-
IC781_RS18765	41620..41904	-	285	WP_032012193.1	antibiotic biosynthesis monooxygenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..82186	82186
	-	inside	IScluster/Tn	-	-	35259..40203	4944

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-106)

Antitoxin

• HigA2-like, helix-turn-helix domain (13-89)

Sequences

Toxin

Protein

• Length: 119 a.a.
• Molecular weight: 13487.44 Da
• Isoelectric Point: 6.4631

>T172761 WP_000269901.1 NZ_CP061627:c37632-37276 [Acinetobacter seifertii]
MWTVITTDLFNEWLAQQDESTQEKVLAALVVLQQQGPSLGRPLVDTVYDSKFTNMKELRVQHRGKPLRAFFAFDPLRQAI
VLCIGDKGGKKRFYKEMLDIADQQYEIHLSTLGDQSNG

Nucleotide

• Length: 357 bp

>T172761 NZ_CP079923:931268-932029 [Streptococcus pneumoniae]
ATGGAAATCCAAGATTATACTGATAGTGAATTCAAACATGCTTTAGCAAGGAATCTTCGTTCACTGACAAGAGGAAAAAA
GTCCAGTAAACAACCTATAGCGATTTTGCTTGGAGGGCAAAGTGGTGCCGGTAAGACTACAATTCATCGTATTAAACAGA
AAGAATTTCAAGGAAATATTGTTATCATAGATGGCGATAGTTTTCGTTCTCAGCATCCACACTATTTAGAACTGCAGCAA
GAATATGGCAAAGATAGCGTTGAATACACCAAAGATTTTGCAGGAAAAATGGTAGAGTCTTTAGTAACAAAATTGAGTAG
TTTGGGATACAATCTTTTGATCGAGGGAACTTTACGAACAATTGATGTTCCAAAGAAAACGGCACAACTCTTGAAAAATA
AGGGATATGAAGTACAATTGGCCTTAATTGCGACAAAGCCTGAATTGTCGTATCTAAGTACTCTTATCCGTTATGAAGAA
CTGTACATTATCAATCCAAATCAAGCACGCGCAACTCCAAAAGAACATCATGATTTCATTGTAAATCATCTAGTTGATAA
CACACGAAAATTGGAAGAACTAGCTATCTTTGAAAGAATTCAAATTTACCAACGAGATAGAAGTTGTGTATATGATTCAA
AAGAAAATACAACTTCAGCAGCAGATGTTCTTCAAGAGTTACTCTTTGGGGAGTGGAGTCAGGTAGAGAAGGAGATGTTG
CAGGTGGGGGAAAAGAGACTTAATGAATTACTTGAAAAATAA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11206.01 Da
• Isoelectric Point: 6.7282

>AT172761 WP_001140619.1 NZ_CP061627:c37283-36981 [Acinetobacter seifertii]
MAKTLQELLANRSPESQARIQQMADELLLETQLHLIREELEISQKELAATLGIKQPSLSAIENRGHDLKISTMKKYVEAM
GGKLRIDVELPTGKHIGFNV

Nucleotide

• Length: 303 bp

>AT172761 NZ_CP079923:930792-931268 [Streptococcus pneumoniae]
ATGATTGGAAAGAACATAAAATCCTTACGTAAAACACATGACTTAACACAACCCGAATTTGCACGAATTATAGGAATTTC
TCGAAATAGCTTGAGTCGTTATGAAAATGGAACTAGTTCAGTCTCTACAGAATTAATTGACATCATTTGTCAGAAGTTTA
ATGTATCTTATGTCGATATTGTAGGAGAAGATAAAATGCTCAATCCTGTTGAAGATTATGAATTGACTTTGAAAATTGAA
ATTGTGAAAGAAAGAGGTGCTAATCTATTATCTCGACTCTATCGTTATCAAGATAGTCAGGGAATTAGCATTGATGATGA
ATCTAATCCTTGGATTTTAATGAGTGATGATCTATCTGACTTGATTCATACGAATATCTATTTAGTAGAAACTTTTGATG
AAATAGAGAGATATAGTGGCTATTTGGATGGAATTGAACGTATGTTAGAGATATCTGAAAAGCGGATGGTAGCTTAA

Structures

Toxin

• AlphaFold DB: A0A0E2G754