Detailed information of TA system TA172760

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/Tad-HTH_37
• Location: 33575..34037
• Replicon: plasmid pAS48-1
• Accession: NZ_CP061627
• Organism: Acinetobacter seifertii strain AS48

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A3R9PS35
• Locus tag: IC781_RS18700
• Protein ID: WP_000033474.1
• Coordinates: 33849..34037 (-)
• Length: 63 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: IC781_RS18695
• Protein ID: WP_001258992.1
• Coordinates: 33575..33862 (-)
• Length: 96 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IC781_RS18655	28955..29443	+	489	WP_191011567.1	hypothetical protein	-
IC781_RS18660	29471..29938	+	468	WP_191011568.1	hypothetical protein	-
IC781_RS18665	29907..30548	+	642	Protein_34	hypothetical protein	-
IC781_RS18670	30608..31150	+	543	Protein_35	DUF2280 domain-containing protein	-
IC781_RS18675	31134..32446	+	1313	Protein_36	terminase	-
IC781_RS18680	32486..33043	+	558	Protein_37	hypothetical protein	-
IC781_RS18685	33067..33291	+	225	WP_004882762.1	hypothetical protein	-
IC781_RS18690	33242..33496	+	255	WP_000236586.1	hypothetical protein	-
IC781_RS18695	33575..33862	-	288	WP_001258992.1	helix-turn-helix transcriptional regulator	Antitoxin
IC781_RS18700	33849..34037	-	189	WP_000033474.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
IC781_RS18705	34314..34634	-	321	WP_000088946.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
IC781_RS18710	34822..35211	+	390	WP_002072923.1	AAA family ATPase	-
IC781_RS18715	35139..35684	+	546	Protein_44	IS5-like element ISAba13 family transposase	-
IC781_RS18720	36444..36671	+	228	WP_000286812.1	hypothetical protein	-
IC781_RS18725	36779..36988	-	210	WP_000069471.1	hypothetical protein	-
IC781_RS18730	36981..37283	-	303	WP_001140619.1	XRE family transcriptional regulator	-
IC781_RS18735	37276..37632	-	357	WP_000269901.1	type II toxin-antitoxin system RelE/ParE family toxin	-
IC781_RS18740	38214..38720	+	507	WP_191011569.1	sel1 repeat family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..82186	82186
	-	inside	IScluster/Tn	-	-	35259..40203	4944

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (2-55)

Antitoxin

• Cro/C1-type helix-turn-helix domain (39-90)

Sequences

Toxin

Protein

• Length: 63 a.a.
• Molecular weight: 7137.56 Da
• Isoelectric Point: 10.9048

>T172760 WP_000033474.1 NZ_CP061627:c34037-33849 [Acinetobacter seifertii]
MSKGLFELRLKSQEGIARVMYCTLVGKRIVMLHSFVKKTQKTPKQDLNLALDRMKEVKNANT

Nucleotide

• Length: 189 bp

>T172760 NZ_CP079922:c1589384-1589205 [Streptococcus pneumoniae]
ATGCCTATGACACAAAAAGAGATGGTTAAACTCCTTACGGCCCATGGTTGGATAAAGACTAGAGGCGGTAAAGGCTCCCA
TATTAAAATGGAAAAGCAAGGGGAAAGACCTATCACAATCCTTCATGGTAAGCTGAATAAGTACACTGAAAGAGGGATAA
GAAAGCAAGCTGGGTTGTAA

Antitoxin

Protein

• Length: 96 a.a.
• Molecular weight: 10563.32 Da
• Isoelectric Point: 9.7308

>AT172760 WP_001258992.1 NZ_CP061627:c33862-33575 [Acinetobacter seifertii]
MRTHEEMKALALSRSAVRTEYERIEREEMPLLDMVLTALREAGLSQAQIAERMGTNVPAVSRLEKALITGKPSPSIATLQ
KYAAAIGKHVEVRFV

Nucleotide

• Length: 288 bp

>AT172760 NZ_CP079922:c1589168-1588716 [Streptococcus pneumoniae]
ATGTTAGTTACGTATCCAGCTTTATTTTATTATGACGACACAGACGGAACAGAAGCGACTTATTTTGTCCATTTCCCAGA
TTTTGAATACTCAGCTACACAAGGAGAGGGGATTTCTGAGGCTTTGGCTATGGGGTCGGAGTGGCTAGGGATAACTGTTG
CAGATTTGATAGAGAGTGATGGAGAGTTACCTCAGCCATCAGATATTAATAGCTTGTCTTTAATTGATAATGATCCTTTT
AAAGATGATGAAGATTTCGTGTCAACCTATGACCTTGATAAATCTTTTATTTCTATGGTATCTGTTGATGTATCAGAATA
CCTAGGAAGTCAGGAACCCATTAAAAAGACTTTGACTATACCAAAATGGGCAGATAAGTTGGGACGAGAAATGGGACTTA
ACTTTTCTCAGACTTTGACAGACGCTATTGCAGATAAGAAAGTTCAAGCCTAA

Structures

Toxin

• AlphaFold DB: A0A3R9PS35