Detailed information of TA system TA172758

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 7437..8095
• Replicon: plasmid pAS49-3
• Accession: NZ_CP061623
• Organism: Acinetobacter seifertii strain AS49

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: IC782_RS21455
• Protein ID: WP_000312250.1
• Coordinates: 7736..8095 (-)
• Length: 120 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: IC782_RS21450
• Protein ID: WP_001096429.1
• Coordinates: 7437..7736 (-)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IC782_RS21415	3496..3753	+	258	WP_000834292.1	hypothetical protein	-
IC782_RS21420	3758..4330	+	573	WP_032011345.1	hypothetical protein	-
IC782_RS21425	4306..4485	+	180	WP_002081921.1	hypothetical protein	-
IC782_RS21430	4502..5131	+	630	WP_002081923.1	hypothetical protein	-
IC782_RS21435	5171..5680	+	510	WP_032018705.1	hypothetical protein	-
IC782_RS21440	5749..6315	+	567	WP_191009757.1	hypothetical protein	-
IC782_RS21445	6365..6901	+	537	WP_032010581.1	hypothetical protein	-
IC782_RS21450	7437..7736	-	300	WP_001096429.1	helix-turn-helix transcriptional regulator	Antitoxin
IC782_RS21455	7736..8095	-	360	WP_000312250.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
IC782_RS21460	8296..8862	+	567	WP_031948745.1	hypothetical protein	-
IC782_RS21465	8911..9141	+	231	WP_045124240.1	hypothetical protein	-
IC782_RS21470	9160..9858	+	699	WP_000873188.1	hypothetical protein	-
IC782_RS21475	9996..10379	+	384	WP_032018724.1	hypothetical protein	-
IC782_RS21480	10446..11204	+	759	WP_001053130.1	hypothetical protein	-
IC782_RS21485	11256..11591	+	336	WP_000654056.1	hypothetical protein	-
IC782_RS21490	12457..12771	+	315	WP_032011240.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	blaOXA-82	-	1..74130	74130

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (14-109)

Antitoxin

• HigA2-like, helix-turn-helix domain (12-88)

Sequences

Toxin

Protein

• Length: 120 a.a.
• Molecular weight: 13751.62 Da
• Isoelectric Point: 4.8212

>T172758 WP_000312250.1 NZ_CP061623:c8095-7736 [Acinetobacter seifertii]
MAWDVETTELFDSWLAEQDENAQDKILASLLVLSELGPNLGRPHVDTIKESKYPNMKEIRVQVKGHPIRGFFAFDPERKA
IVLCAGDKKGLNEKAFYKEMIKIADEQYEQYLRDNYGDK

Nucleotide

• Length: 360 bp

>T172758 NZ_CP079922:c1106487-1106233 [Streptococcus pneumoniae]
TTGTATAAATTAGTTCCAACAAGACGTTTCATCAAGCAATTGAAAAAATTGGACCGTTATACGCAGAAGCTAATTACAAA
CTATTTACAAACCAATGTTTTGGAAGACCCAAGACGACACGGAAAGGCTTTGGTTGGTAATCGCGTTGGTCAATGGCGCT
ATAGAATTGGTAATTATCGAGTTATCGTACAAATTGTAGATGATGAATTAGTCGTTGCTACTCTAGAAGTTGGTCATCGG
AGAGATATTTATTGA

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 11012.93 Da
• Isoelectric Point: 10.4856

>AT172758 WP_001096429.1 NZ_CP061623:c7736-7437 [Acinetobacter seifertii]
MAKLNDIISKRSPESQQRIKKMANQMLLDINLSNLREELKISQAEIAKNLGVSQAAISKREKLGKDLKISSLKEYVEAMG
GKVRLDVELPNGKHVAISI

Nucleotide

• Length: 300 bp

>AT172758 NZ_CP079922:c1106731-1106489 [Streptococcus pneumoniae]
ATGACTACTATTACATTAAAAGTTTCTGAAGCTGATAAAACATTTATGAAAGCAATGGCTAAGTTTGAAGGAGTTTCCCT
TTCGGAACTTATTCGCACCAAAACTCTTGAAGCCCTAGAAGATGAATACGATGCTCGTGTGGCAGATTTAGCCTATCAAG
AGTATTTAGAAGACTTGGAAAAAGGAGTTGAACCCATTACTTGGGAAGAAATGATGCATGATTTAGGCTTGAAGGATGAA
TAA