Detailed information of TA system TA172757

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 58269..58920
• Replicon: plasmid pAS49-2
• Accession: NZ_CP061622
• Organism: Acinetobacter seifertii strain AS49

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A7H2WXC0
• Locus tag: IC782_RS21125
• Protein ID: WP_006581604.1
• Coordinates: 58269..58625 (+)
• Length: 119 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: IC782_RS21130
• Protein ID: WP_001140619.1
• Coordinates: 58618..58920 (+)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IC782_RS21105	53726..54934	-	1209	WP_004832869.1	IS256-like element ISAba26 family transposase	-
IC782_RS21110	55219..55395	+	177	WP_006581600.1	hypothetical protein	-
IC782_RS21115	55429..55791	+	363	Protein_58	DNA-binding protein	-
IC782_RS21120	56005..57459	-	1455	WP_062032131.1	SulP family inorganic anion transporter	-
IC782_RS21125	58269..58625	+	357	WP_006581604.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
IC782_RS21130	58618..58920	+	303	WP_001140619.1	XRE family transcriptional regulator	Antitoxin
IC782_RS21135	58913..59122	+	210	WP_000069471.1	hypothetical protein	-
IC782_RS21140	59232..59450	-	219	WP_029747731.1	hypothetical protein	-
IC782_RS21150	61360..61673	-	314	Protein_65	AAA family ATPase	-
IC782_RS21155	61859..62179	+	321	WP_002123990.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
IC782_RS21160	62456..62644	+	189	WP_000033474.1	type II toxin-antitoxin system RelE/ParE family toxin	-
IC782_RS21165	62631..62920	+	290	Protein_68	helix-turn-helix domain-containing protein	-
IC782_RS21170	62999..63253	-	255	WP_000236586.1	hypothetical protein	-
IC782_RS21175	63204..63501	-	298	Protein_70	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..105812	105812
	-	flank	IS/Tn	-	-	53726..54934	1208

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-106)

Antitoxin

• HigA2-like, helix-turn-helix domain (13-89)

Sequences

Toxin

Protein

• Length: 119 a.a.
• Molecular weight: 13462.38 Da
• Isoelectric Point: 7.1642

>T172757 WP_006581604.1 NZ_CP061622:58269-58625 [Acinetobacter seifertii]
MWTVITTDLFNEWLAQQDQSTQEKVLAALVVLQQQGPSLGRPLVDTVYDSKFTNMKDLRVQHRGKSLRAFFAFDPLRQAI
VLCIGDKGGKKRFYKEMLDIADQQYEIHLSTLGDQSNG

Nucleotide

• Length: 357 bp

>T172757 NZ_CP079922:936730-937491 [Streptococcus pneumoniae]
ATGGAAATCCAAGATTATACTGATAGTGAATTCAAACATGCTTTAGCAAGGAATCTTCGTTCACTGACAAGAGGAAAAAA
GTCCAGTAAACAACCTATAGCGATTTTGCTTGGAGGGCAAAGTGGTGCCGGTAAGACTACAATTCATCGTATTAAACAGA
AAGAATTTCAAGGAAATATTGTTATCATAGATGGCGATAGTTTTCGTTCTCAGCATCCACACTATTTAGAACTGCAGCAA
GAATATGGCAAAGATAGCGTTGAATACACCAAAGATTTTGCAGGAAAAATGGTAGAGTCTTTAGTAACAAAATTGAGTAG
TTTGGGATACAATCTTTTGATCGAGGGAACTTTACGAACAATTGATGTTCCAAAGAAAACGGCACAACTCTTGAAAAATA
AGGGATATGAAGTACAATTGGCCTTAATTGCGACAAAGCCTGAATTGTCGTATCTAAGTACTCTTATCCGTTATGAAGAA
CTGTACATTATCAATCCAAATCAAGCACGCGCAACTCCAAAAGAACATCATGATTTCATTGTAAATCATCTAGTTGATAA
CACACGAAAATTGGAAGAACTAGCTATCTTTGAAAGAATTCAAATTTACCAACGAGATAGAAGTTGTGTATATGATTCAA
AAGAAAATACAACTTCAGCAGCAGATGTTCTTCAAGAGTTACTCTTTGGGGAGTGGAGTCAGGTAGAGAAGGAGATGTTG
CAGGTGGGGGAAAAGAGACTTAATGAATTACTTGAAAAATAA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11206.01 Da
• Isoelectric Point: 6.7282

>AT172757 WP_001140619.1 NZ_CP061622:58618-58920 [Acinetobacter seifertii]
MAKTLQELLANRSPESQARIQQMADELLLETQLHLIREELEISQKELAATLGIKQPSLSAIENRGHDLKISTMKKYVEAM
GGKLRIDVELPTGKHIGFNV

Nucleotide

• Length: 303 bp

>AT172757 NZ_CP079922:936254-936730 [Streptococcus pneumoniae]
ATGATTGGAAAGAACATAAAATCCTTACGTAAAACACATGACTTAACACAACCCGAATTTGCACGAATTATAGGAATTTC
TCGAAATAGCTTGAGTCGTTATGAAAATGGAACTAGTTCAGTCTCTACAGAATTAATTGACATCATTTGTCAGAAGTTTA
ATGTATCTTATGTCGATATTGTAGGAGAAGATAAAATGCTCAATCCTGTTGAAGATTATGAATTGACTTTGAAAATTGAA
ATTGTGAAAGAAAGAGGTGCTAATCTATTATCTCGACTCTATCGTTATCAAGATAGTCAGGGAATTAGCATTGATGATGA
ATCTAATCCTTGGATTTTAATGAGTGATGATCTATCTGACTTGATTCATACGAATATCTATTTAGTAGAAACTTTTGATG
AAATAGAGAGATATAGTGGCTATTTGGATGGAATTGAACGTATGTTAGAGATATCTGAAAAGCGGATGGTAGCTTAA

Structures

Toxin

• AlphaFold DB: A0A7H2WXC0