Detailed information of TA system TA172754

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 87332..87983
• Replicon: plasmid pAS51-2
• Accession: NZ_CP061615
• Organism: Acinetobacter seifertii strain AS51

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A7H2WXC0
• Locus tag: IC783_RS21670
• Protein ID: WP_006581604.1
• Coordinates: 87627..87983 (-)
• Length: 119 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: IC783_RS21665
• Protein ID: WP_001140619.1
• Coordinates: 87332..87634 (-)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IC783_RS21620	82751..83048	+	298	Protein_85	hypothetical protein	-
IC783_RS21625	82999..83253	+	255	WP_000236586.1	hypothetical protein	-
IC783_RS21630	83332..83621	-	290	Protein_87	helix-turn-helix domain-containing protein	-
IC783_RS21635	83608..83796	-	189	WP_000033474.1	type II toxin-antitoxin system RelE/ParE family toxin	-
IC783_RS21640	84073..84393	-	321	WP_002123990.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
IC783_RS21645	84579..84892	+	314	Protein_90	AAA family ATPase	-
IC783_RS21650	84972..86131	+	1160	WP_085945698.1	IS3 family transposase	-
IC783_RS21655	86802..87020	+	219	WP_029747731.1	hypothetical protein	-
IC783_RS21660	87130..87339	-	210	WP_000069471.1	hypothetical protein	-
IC783_RS21665	87332..87634	-	303	WP_001140619.1	XRE family transcriptional regulator	Antitoxin
IC783_RS21670	87627..87983	-	357	WP_006581604.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
IC783_RS21675	88793..90247	+	1455	WP_062032131.1	SulP family inorganic anion transporter	-
IC783_RS21680	90461..90823	-	363	Protein_97	DNA-binding protein	-
IC783_RS21685	90857..91033	-	177	WP_006581600.1	hypothetical protein	-
IC783_RS21690	91318..92526	+	1209	WP_004832869.1	IS256-like element ISAba26 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..105861	105861
	-	flank	IS/Tn	-	-	91318..92526	1208

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-106)

Antitoxin

• HigA2-like, helix-turn-helix domain (13-89)

Sequences

Toxin

Protein

• Length: 119 a.a.
• Molecular weight: 13462.38 Da
• Isoelectric Point: 7.1642

>T172754 WP_006581604.1 NZ_CP061615:c87983-87627 [Acinetobacter seifertii]
MWTVITTDLFNEWLAQQDQSTQEKVLAALVVLQQQGPSLGRPLVDTVYDSKFTNMKDLRVQHRGKSLRAFFAFDPLRQAI
VLCIGDKGGKKRFYKEMLDIADQQYEIHLSTLGDQSNG

Nucleotide

• Length: 357 bp

>T172754 NZ_CP079921:1683546-1683911 [Actinobacillus pleuropneumoniae]
ATGGATACCAATGTACGACAATGGTTAGCAACGATTAAACCGAGTCAGACGAATATTAGCTGTATCACGCTTTCCGAAAT
AAAAACGGGGATTTTACTCAAAGCTCGTAAAGATCCTATTCAAGCCGAGCGGTTAAATCATTGGTTTACCCATAATGTAT
TAAGCGTTTATCAAGCTAAATCCTTTTCTATAAATAATGAAATTGCGTTACTGGCTTCTGAATACCACATTCCAAATAAA
ATGGATTTAAACGATGCTTATATTGCCGCAACCGCTAAATATCATAATTTAGTGCTTGTTACTCGTAATTTGAAAGACTT
CAACCGTTGCGATATACGTTTATTTAATCCTTTCGAACCGAATTAA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11206.01 Da
• Isoelectric Point: 6.7282

>AT172754 WP_001140619.1 NZ_CP061615:c87634-87332 [Acinetobacter seifertii]
MAKTLQELLANRSPESQARIQQMADELLLETQLHLIREELEISQKELAATLGIKQPSLSAIENRGHDLKISTMKKYVEAM
GGKLRIDVELPTGKHIGFNV

Nucleotide

• Length: 303 bp

>AT172754 NZ_CP079921:1683196-1683483 [Actinobacillus pleuropneumoniae]
ATGCCAATTACTATTACCTCTCGAGAATTTAATCAACACGCCAGTAAAGTGCTGAAAATGGCAGAAAAAGAACCGGTTTT
TATCACTAAATGGGGAAAAATTGTCAGCGTTGTCTCCAATTATGTTGATTACAAACAGCACACAACGGTTGAGCCTAGCT
TTGAAGAACTATTTGCCTCATCTACGGATGAATCGGTTAGCGATGAGTTTTGGCAAGATTTTGAACAGACATTAACAAAA
ATTCGCAAAAGTAATAAATCCGAACAAATAGATTTTGGAGATGAATAA

Structures

Toxin

• AlphaFold DB: A0A7H2WXC0