Detailed information of TA system TA172752

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 9854..10505
• Replicon: plasmid pAS53-2
• Accession: NZ_CP061605
• Organism: Acinetobacter seifertii strain AS53

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A7H2WXC0
• Locus tag: IC785_RS21240
• Protein ID: WP_006581604.1
• Coordinates: 10149..10505 (-)
• Length: 119 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: IC785_RS21235
• Protein ID: WP_001140619.1
• Coordinates: 9854..10156 (-)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IC785_RS21190	5273..5570	+	298	Protein_6	hypothetical protein	-
IC785_RS21195	5521..5775	+	255	WP_000236586.1	hypothetical protein	-
IC785_RS21200	5854..6143	-	290	Protein_8	helix-turn-helix domain-containing protein	-
IC785_RS21205	6130..6318	-	189	WP_000033474.1	type II toxin-antitoxin system RelE/ParE family toxin	-
IC785_RS21210	6595..6915	-	321	WP_002123990.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
IC785_RS21215	7101..7414	+	314	Protein_11	AAA family ATPase	-
IC785_RS21220	7494..8653	+	1160	WP_085945698.1	IS3 family transposase	-
IC785_RS21225	9324..9542	+	219	WP_029747731.1	hypothetical protein	-
IC785_RS21230	9652..9861	-	210	WP_000069471.1	hypothetical protein	-
IC785_RS21235	9854..10156	-	303	WP_001140619.1	XRE family transcriptional regulator	Antitoxin
IC785_RS21240	10149..10505	-	357	WP_006581604.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
IC785_RS21245	11315..12769	+	1455	WP_062032131.1	SulP family inorganic anion transporter	-
IC785_RS21250	12983..13345	-	363	Protein_18	DNA-binding protein	-
IC785_RS21255	13379..13603	-	225	WP_125510319.1	hypothetical protein	-
IC785_RS21260	13840..15048	+	1209	WP_004832869.1	IS256-like element ISAba26 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..105818	105818
	-	flank	IS/Tn	-	-	13840..15048	1208

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-106)

Antitoxin

• HigA2-like, helix-turn-helix domain (13-89)

Sequences

Toxin

Protein

• Length: 119 a.a.
• Molecular weight: 13462.38 Da
• Isoelectric Point: 7.1642

>T172752 WP_006581604.1 NZ_CP061605:c10505-10149 [Acinetobacter seifertii]
MWTVITTDLFNEWLAQQDQSTQEKVLAALVVLQQQGPSLGRPLVDTVYDSKFTNMKDLRVQHRGKSLRAFFAFDPLRQAI
VLCIGDKGGKKRFYKEMLDIADQQYEIHLSTLGDQSNG

Nucleotide

• Length: 357 bp

>T172752 NZ_CP079921:1441994-1442254 [Actinobacillus pleuropneumoniae]
ATGAAGCTAACTTTCTCGTCTAATGCGTGGGAAGATTATTTATACTGGCAAAAAACCGATAAAATAATTTTGAAACGTAT
AAACAGCCTGATCAAAGATATTCAACGCCAACCATTCGAAGGCATAGGAAAACTGGAGCCGCTAAAATTCAATCTTTCGG
GATTTTGGTCAAGGCGAATCAATGAAGAACACCGCTTAATTTACAGCGTGGAAGATGAAGCAATTTTAATAGTAGCGTGT
AGATACCATTATGATCAGTAA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11206.01 Da
• Isoelectric Point: 6.7282

>AT172752 WP_001140619.1 NZ_CP061605:c10156-9854 [Acinetobacter seifertii]
MAKTLQELLANRSPESQARIQQMADELLLETQLHLIREELEISQKELAATLGIKQPSLSAIENRGHDLKISTMKKYVEAM
GGKLRIDVELPTGKHIGFNV

Nucleotide

• Length: 303 bp

>AT172752 NZ_CP079921:1441746-1441997 [Actinobacillus pleuropneumoniae]
ATGCAAGCAATAACCTACACAGAAGCACGCCAAAATCTAGCCGGTACGATGTCAAAAGTAGCAGAAGATTTTGAGCCTAT
TTTAATTACAAGAAGCAAGGGCGGAAATTGCGTACTTATGTCGTATGAGCAGTATTGTTCACTTGAAGAAACCGCCTATT
TGATGCGTTCACCCACCAATGCAAAACGGCTATTGGATAGTGTGGCGGAATTAAGACAGGGACAAGGCATTGAAAGAGAG
CTAATCGAATGA

Structures

Toxin

• AlphaFold DB: A0A7H2WXC0