Detailed information of TA system TA172747

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 35471..36129
• Replicon: plasmid pAS62-3
• Accession: NZ_CP061585
• Organism: Acinetobacter seifertii strain AS62

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: IC791_RS21095
• Protein ID: WP_000312250.1
• Coordinates: 35770..36129 (-)
• Length: 120 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: IC791_RS21090
• Protein ID: WP_001096429.1
• Coordinates: 35471..35770 (-)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IC791_RS21055	31530..31787	+	258	WP_000834292.1	hypothetical protein	-
IC791_RS21060	31792..32364	+	573	WP_032011345.1	hypothetical protein	-
IC791_RS21065	32340..32519	+	180	WP_002081921.1	hypothetical protein	-
IC791_RS21070	32536..33165	+	630	WP_002081923.1	hypothetical protein	-
IC791_RS21075	33205..33714	+	510	WP_032018705.1	hypothetical protein	-
IC791_RS21080	33795..34349	+	555	WP_000790086.1	hypothetical protein	-
IC791_RS21085	34399..34935	+	537	WP_032010581.1	hypothetical protein	-
IC791_RS21090	35471..35770	-	300	WP_001096429.1	helix-turn-helix transcriptional regulator	Antitoxin
IC791_RS21095	35770..36129	-	360	WP_000312250.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
IC791_RS21100	36330..36896	+	567	WP_031948745.1	hypothetical protein	-
IC791_RS21105	36945..37175	+	231	WP_045124240.1	hypothetical protein	-
IC791_RS21110	37194..37893	+	700	Protein_54	hypothetical protein	-
IC791_RS21115	38031..38414	+	384	WP_032018724.1	hypothetical protein	-
IC791_RS21120	38481..39239	+	759	WP_001053130.1	hypothetical protein	-
IC791_RS21125	39291..39626	+	336	WP_000654056.1	hypothetical protein	-
IC791_RS21130	40491..40805	+	315	WP_032011240.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	blaOXA-82	-	1..74124	74124

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (14-109)

Antitoxin

• HigA2-like, helix-turn-helix domain (12-88)

Sequences

Toxin

Protein

• Length: 120 a.a.
• Molecular weight: 13751.62 Da
• Isoelectric Point: 4.8212

>T172747 WP_000312250.1 NZ_CP061585:c36129-35770 [Acinetobacter seifertii]
MAWDVETTELFDSWLAEQDENAQDKILASLLVLSELGPNLGRPHVDTIKESKYPNMKEIRVQVKGHPIRGFFAFDPERKA
IVLCAGDKKGLNEKAFYKEMIKIADEQYEQYLRDNYGDK

Nucleotide

• Length: 360 bp

>T172747 NZ_CP079894:14139-14420 [Escherichia fergusonii]
ATGACCTATGAACTGGCTTTTGACCGTAGAGCACTGAAGGAATGGCAGAAACTCGGCCACACCATCCGTGAACAATTCAA
AAAGAAACTGGCAGAACGGCTGGAAAATCCACGCGTACCCGCAGCCCGGTTACATGGTCATGCTGATCGCTATAAAATCA
AACTTCGTGCATCTGGCTACAGACTTGTATACCAAGTCATTGATGAGAAAGTCGTTTTACTCGTTATTGCCGTTGGAAGA
AGGGAAAGCAGCGAAGTCTATCAGATCGCAGATTTGCGCTAA

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 11012.93 Da
• Isoelectric Point: 10.4856

>AT172747 WP_001096429.1 NZ_CP061585:c35770-35471 [Acinetobacter seifertii]
MAKLNDIISKRSPESQQRIKKMANQMLLDINLSNLREELKISQAEIAKNLGVSQAAISKREKLGKDLKISSLKEYVEAMG
GKVRLDVELPNGKHVAISI

Nucleotide

• Length: 300 bp

>AT172747 NZ_CP079894:13898-14149 [Escherichia fergusonii]
ATGAGCTATCAAATTCTGACAACCACAGCGGCCAGTATTACTGACCTGAAAAAAAATCCTATGGGAACCGTAGCTGAAGG
TGAAGGGGACGCTGTTGCGATCCTGAACCGAAACGAACCGGCGTTCTATTGCGTTCCACCAAAACTTTACGCCTACTATC
GGGAACTCGCTGAAGATGCTGAGTTAAACGCTGTTGCTGATGAGCGCATGAAAAACCCGGAAATTGTGAAGGTTAACCTG
GATGACCTATGA