Detailed information of TA system TA172744

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 9856..10507
• Replicon: plasmid pAS63-1
• Accession: NZ_CP061579
• Organism: Acinetobacter seifertii strain AS63

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A7H2WXC0
• Locus tag: IC792_RS20000
• Protein ID: WP_006581604.1
• Coordinates: 10151..10507 (-)
• Length: 119 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: IC792_RS19995
• Protein ID: WP_001140619.1
• Coordinates: 9856..10158 (-)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IC792_RS19950	5275..5572	+	298	Protein_6	hypothetical protein	-
IC792_RS19955	5523..5777	+	255	WP_000236586.1	hypothetical protein	-
IC792_RS19960	5856..6145	-	290	Protein_8	helix-turn-helix domain-containing protein	-
IC792_RS19965	6132..6320	-	189	WP_000033474.1	type II toxin-antitoxin system RelE/ParE family toxin	-
IC792_RS19970	6597..6917	-	321	WP_002123990.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
IC792_RS19975	7103..7416	+	314	Protein_11	AAA family ATPase	-
IC792_RS19980	7496..8655	+	1160	WP_085945698.1	IS3 family transposase	-
IC792_RS19985	9326..9544	+	219	WP_029747731.1	hypothetical protein	-
IC792_RS19990	9654..9863	-	210	WP_000069471.1	hypothetical protein	-
IC792_RS19995	9856..10158	-	303	WP_001140619.1	XRE family transcriptional regulator	Antitoxin
IC792_RS20000	10151..10507	-	357	WP_006581604.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
IC792_RS20005	11317..12771	+	1455	WP_062032131.1	SulP family inorganic anion transporter	-
IC792_RS20010	12985..13347	-	363	Protein_18	DNA-binding protein	-
IC792_RS20015	13381..13557	-	177	WP_006581600.1	hypothetical protein	-
IC792_RS20020	13842..15050	+	1209	WP_004832869.1	IS256-like element ISAba26 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..105830	105830
	-	flank	IS/Tn	-	-	13842..15050	1208

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-106)

Antitoxin

• HigA2-like, helix-turn-helix domain (13-89)

Sequences

Toxin

Protein

• Length: 119 a.a.
• Molecular weight: 13462.38 Da
• Isoelectric Point: 7.1642

>T172744 WP_006581604.1 NZ_CP061579:c10507-10151 [Acinetobacter seifertii]
MWTVITTDLFNEWLAQQDQSTQEKVLAALVVLQQQGPSLGRPLVDTVYDSKFTNMKDLRVQHRGKSLRAFFAFDPLRQAI
VLCIGDKGGKKRFYKEMLDIADQQYEIHLSTLGDQSNG

Nucleotide

• Length: 357 bp

>T172744 NZ_CP079893:7056-7310 [Escherichia fergusonii]
ATGAGAAAAAACAAAGGGCGATTGACTTATTATCTTGAAGTGATTGATAAAAAATACCATTTTGTAAAAAAAATAAGCAG
TTATTCAAAGGAGTTCACTGACGGAAAAACAAAAAGAACAAAGAGAACGTTAAGTGAGCTGGTTTTTAATGAAAGTGAGG
TCGAGGCAATAGACTTTACAAAAAATGGTTTAAGACCTGTTGATAAGAATATTCTCTTAACTATGGTGAAAGAATATAAG
GAGAGTGATGCATGA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11206.01 Da
• Isoelectric Point: 6.7282

>AT172744 WP_001140619.1 NZ_CP061579:c10158-9856 [Acinetobacter seifertii]
MAKTLQELLANRSPESQARIQQMADELLLETQLHLIREELEISQKELAATLGIKQPSLSAIENRGHDLKISTMKKYVEAM
GGKLRIDVELPTGKHIGFNV

Nucleotide

• Length: 303 bp

>AT172744 NZ_CP079893:7307-7549 [Escherichia fergusonii]
ATGACTATTGAAAATACACCGGAAAACATAAAGAAATTACGTAAAAAAATTGGCCTTACCCAGACAGAGTGCGGTGAGAT
TTTTGGCGTAGGCTTAAGTACATGGCAGAAAAAAGAAGCTAAAACCCACAACCAGCTTAATTTATCGAAAGGTGAATTTG
AGTACCTGTTATTACTTGCAGGGGAGCACCCTGAATACGTCCTGCATAAAAAAGCTGAATCCCGCTCACCGGAGGAACCG
TGA

Structures

Toxin

• AlphaFold DB: A0A7H2WXC0