Detailed information of TA system TA172741

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 23580..24231
• Replicon: plasmid pAS73-1
• Accession: NZ_CP061562
• Organism: Acinetobacter seifertii strain AS73

Toxin (Protein)

• Gene name: higB
• Uniprot ID: S3SZA7
• Locus tag: IC796_RS19680
• Protein ID: WP_000269903.1
• Coordinates: 23875..24231 (-)
• Length: 119 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: IC796_RS19675
• Protein ID: WP_191036795.1
• Coordinates: 23580..23882 (-)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IC796_RS19635	18996..20204	-	1209	WP_000343018.1	IS256-like element ISAba26 family transposase	-
IC796_RS19640	20267..20464	-	198	WP_151685370.1	DNA-binding protein	-
IC796_RS19645	20451..20639	-	189	WP_000033474.1	type II toxin-antitoxin system RelE/ParE family toxin	-
IC796_RS19650	20916..21236	-	321	WP_002123990.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
IC796_RS19655	21422..21738	+	317	Protein_24	AAA family ATPase	-
IC796_RS19660	21738..22283	+	546	Protein_25	IS5 family transposase	-
IC796_RS19665	23041..23268	+	228	WP_005071544.1	hypothetical protein	-
IC796_RS19670	23378..23587	-	210	WP_000069471.1	hypothetical protein	-
IC796_RS19675	23580..23882	-	303	WP_191036795.1	XRE family transcriptional regulator	Antitoxin
IC796_RS19680	23875..24231	-	357	WP_000269903.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
IC796_RS19685	24756..25946	-	1191	WP_005144704.1	hypothetical protein	-
IC796_RS19690	26154..26516	-	363	Protein_31	DNA-binding protein	-
IC796_RS19695	26550..26774	-	225	WP_126026108.1	hypothetical protein	-
IC796_RS19700	26924..27019	-	96	Protein_33	DNA-binding protein	-
IC796_RS19705	27017..27187	-	171	Protein_34	IS3 family transposase	-
IC796_RS19710	27363..27647	-	285	WP_005071539.1	antibiotic biosynthesis monooxygenase	-
IC796_RS19715	27667..28665	-	999	WP_005071537.1	zinc-binding alcohol dehydrogenase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..113715	113715
	-	inside	IScluster/Tn	-	-	18996..22283	3287

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-106)

Antitoxin

• HigA2-like, helix-turn-helix domain (13-89)

Sequences

Toxin

Protein

• Length: 119 a.a.
• Molecular weight: 13486.45 Da
• Isoelectric Point: 7.1645

>T172741 WP_000269903.1 NZ_CP061562:c24231-23875 [Acinetobacter seifertii]
MWTVITTDLFNEWLAQQDQSTQEKVLAALVVLQQQGPSLGRPLVDTVYDSKFTNMKELRVQHRGKPLRAFFAFDPLRQAI
VLCIGDKGGKKRFYKEMLDIADQQYEIHLSTLGDQSNG

Nucleotide

• Length: 357 bp

>T172741 NZ_CP079891:4165611-4165763 [Escherichia fergusonii]
ATGCTGACAAAATATGCCCTTGTGGCAATCATCGTACTGTGTTGTACAGTACTGGGATTCACGCTGATGGTAGGCGACTC
GTTGTGTGAGTTGAGTATCAGAGAACGTGGTATGGAGTTTAAGGCAGTTCTCGCTTACGAATCGAAGAAGTAG

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11133.94 Da
• Isoelectric Point: 7.3196

>AT172741 WP_191036795.1 NZ_CP061562:c23882-23580 [Acinetobacter seifertii]
MAKTLQELLANRSPESQARIQQMADELLLETQLHLIREELEISQKELAATLGIKQPSLSAIENRGHDLKISTMKKYVGAM
GGKLRIDVELPTGKHIGFNV

Nucleotide

• Length: 303 bp

>AT172741 NZ_CP079891:c4165569-4165517 [Escherichia fergusonii]
TTAGGTTCGAACGCTGGCCTCAGGTTGATAGAAATATCGCCTGGGGCTTTTGT

Structures

Toxin

• AlphaFold DB: A0A836MG83