Detailed information of TA system TA172740

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/Tad(toxin)
• Location: 20267..20639
• Replicon: plasmid pAS73-1
• Accession: NZ_CP061562
• Organism: Acinetobacter seifertii strain AS73

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A3R9PS35
• Locus tag: IC796_RS19645
• Protein ID: WP_000033474.1
• Coordinates: 20451..20639 (-)
• Length: 63 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: IC796_RS19640
• Protein ID: WP_151685370.1
• Coordinates: 20267..20464 (-)
• Length: 66 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IC796_RS19615	15881..16422	+	542	Protein_16	DUF2280 domain-containing protein	-
IC796_RS19620	16406..17722	+	1317	WP_005071556.1	hypothetical protein	-
IC796_RS19625	17762..18319	+	558	Protein_18	hypothetical protein	-
IC796_RS19630	18343..18567	+	225	WP_016653391.1	hypothetical protein	-
IC796_RS19635	18996..20204	-	1209	WP_000343018.1	IS256-like element ISAba26 family transposase	-
IC796_RS19640	20267..20464	-	198	WP_151685370.1	DNA-binding protein	Antitoxin
IC796_RS19645	20451..20639	-	189	WP_000033474.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
IC796_RS19650	20916..21236	-	321	WP_002123990.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
IC796_RS19655	21422..21738	+	317	Protein_24	AAA family ATPase	-
IC796_RS19660	21738..22283	+	546	Protein_25	IS5 family transposase	-
IC796_RS19665	23041..23268	+	228	WP_005071544.1	hypothetical protein	-
IC796_RS19670	23378..23587	-	210	WP_000069471.1	hypothetical protein	-
IC796_RS19675	23580..23882	-	303	WP_191036795.1	XRE family transcriptional regulator	-
IC796_RS19680	23875..24231	-	357	WP_000269903.1	type II toxin-antitoxin system RelE/ParE family toxin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..113715	113715
	-	inside	IScluster/Tn	-	-	18996..22283	3287

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (2-55)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 63 a.a.
• Molecular weight: 7137.56 Da
• Isoelectric Point: 10.9048

>T172740 WP_000033474.1 NZ_CP061562:c20639-20451 [Acinetobacter seifertii]
MSKGLFELRLKSQEGIARVMYCTLVGKRIVMLHSFVKKTQKTPKQDLNLALDRMKEVKNANT

Nucleotide

• Length: 189 bp

>T172740 NZ_CP079891:4149048-4149383 [Escherichia fergusonii]
ATGGTAAGCCGATACGTACCCGATACGGGCGATCTGATTTGGGTTGATTTTGACCCGACAAAAGGTAGCGAGCAAGCCGG
ACATCGTCCGGCTGTTGTCCTGAGTCCGTTCATGTACAACAACAAAACAGGTATGTGTCTGTGTGTTCCTTGTACAACGC
AATCAAAAGGATATCCGTTCGAAGTTGTTTTATCCGGTCAGGAACGTGATGGTGTAGCGTTAGCTGATCAGGTAAAAAGT
ATCGCCTGGCGGGCAAGAGGAGCAACGAAGAAAGGAACGGTTGCCCCAGAGGAATTACAACTCATTAAAGCCAAAATTAA
CGTACTGATTGGGTAG

Antitoxin

Protein

• Length: 66 a.a.
• Molecular weight: 7361.52 Da
• Isoelectric Point: 5.8109

>AT172740 WP_151685370.1 NZ_CP061562:c20464-20267 [Acinetobacter seifertii]
MRTHEEMKALALSRSAVRTEYERIEREEMPLLDMVLTALREAGLSQAQIAERMGTNVPAVSRGLF

Nucleotide

• Length: 198 bp

>AT172740 NZ_CP079891:4148800-4149048 [Escherichia fergusonii]
ATGATCCACAGTAGCGTAAAGCGTTGGGGAAATTCTCCTGCGGTGCGGATCCCGGCTACGTTAATGCAGGCGCTCAATCT
GAATATTGATGATGAAGTGAAGATTGACCTGGTGGATGGCAAATTAATTATTGAGCCAGTGCGTAAAGAGCCCGTATTTA
CGCTTGCTGAACTGGTCAACGACATCACGCCGGAAAACCTCCACGAGAATATCGACTGGGGAGAGCCGAAAGATAAGGAA
GTCTGGTAA

Structures

Toxin

• AlphaFold DB: A0A3R9PS35