Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 3842976..3843775 Replicon chromosome
Accession NZ_CP061530
Organism Escherichia coli strain WEM25

Toxin (Protein)


Gene name yhaV Uniprot ID V0SSH7
Locus tag H5V41_RS18685 Protein ID WP_000347273.1
Coordinates 3843311..3843775 (+) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag H5V41_RS18680 Protein ID WP_001307405.1
Coordinates 3842976..3843311 (+) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H5V41_RS18665 3838761..3839531 - 771 WP_001058209.1 2-dehydro-3-deoxyglucarate aldolase -
H5V41_RS18670 3839547..3840881 - 1335 WP_190811987.1 galactarate/glucarate/glycerate transporter GarP -
H5V41_RS18675 3841256..3842827 + 1572 WP_001273753.1 galactarate dehydratase -
H5V41_RS18680 3842976..3843311 + 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
H5V41_RS18685 3843311..3843775 + 465 WP_000347273.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
H5V41_RS18690 3843830..3844639 - 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
H5V41_RS18695 3844888..3846168 + 1281 WP_000681920.1 tagatose-bisphosphate aldolase subunit KbaZ -
H5V41_RS18700 3846191..3846664 + 474 WP_001336162.1 PTS N-acetylgalactosamine transporter subunit IIB -
H5V41_RS18705 3846675..3847454 + 780 WP_190811988.1 PTS mannose/fructose/sorbose/N-acetylgalactosamine transporter subunit IIC -
H5V41_RS18710 3847444..3848322 + 879 WP_001295548.1 PTS system mannose/fructose/sorbose family transporter subunit IID -
H5V41_RS18715 3848340..3848774 + 435 WP_180557452.1 PTS sugar transporter subunit IIA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17836.25 Da        Isoelectric Point: 9.6924

>T172731 WP_000347273.1 NZ_CP061530:3843311-3843775 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH

Download         Length: 465 bp

>T172731 NZ_CP079891:c2382359-2382072 [Escherichia fergusonii]
ATGCCAATGGAGTTTGAATGGGATGCAAACAAGGCCAAAAGTAATCGGGTGAAACATGGCATCCGCTTTGAAGATGCGGT
GTTAGTGTTTGATGATCCACAACATTTGTCACAGCAAGATCGCATAGAAAACGGTGAGTATCGCTGGCAGACAATCGGCC
TGGTTTACGGCATCGTGGTTATTCTGGTTGCGCATACCATCCGTTTCGAAAGCGGAAATGAAATTATTCGAATCATCAGT
GCACGAAAAGCAGATCGTAAAGAGAGGAATCGCTATGAGCATGGTTAA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT172731 WP_001307405.1 NZ_CP061530:3842976-3843311 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT172731 NZ_CP079891:c2382085-2381783 [Escherichia fergusonii]
ATGAGCATGGTTAAACATAAACGAGGGACCTCCTCTACACTCGATGTGCAGCATGAGGCCGAACTGAAGGCGCTGGCGAA
TAAATCCGACGAAGATATTGATTACAGTGATATCCCACCTTCCAGCGATGAGCAATGGTCTAACGCAGAACGCGGTAAGT
TTTATCGGCCATTGAAAACTCAGGCTTCGGTGCGTATTGATGCTGATGTGATGGAATGGTTGAAGCGACCAGGTAAAGGC
TATCAAACTCGCCTGAACGCAATTTTACGTGAAGCGATGCTGTGCGATCAGAATAAAAAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0SSH7

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References