Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 2681764..2682266 Replicon chromosome
Accession NZ_CP061530
Organism Escherichia coli strain WEM25

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag H5V41_RS13270 Protein ID WP_000767829.1
Coordinates 2681764..2682018 (-) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag H5V41_RS13275 Protein ID WP_001259255.1
Coordinates 2682015..2682266 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H5V41_RS13240 2676781..2677008 - 228 WP_000985273.1 sulfurtransferase TusA family protein -
H5V41_RS13245 2677022..2678079 - 1058 Protein_2583 transport protein YeeE -
H5V41_RS13250 2678257..2679615 - 1359 WP_190811886.1 putrescine/proton symporter PlaP -
H5V41_RS13255 2679605..2679667 - 63 WP_010723108.1 membrane protein YoeI -
H5V41_RS13260 2679882..2680811 - 930 WP_000803351.1 LysR family transcriptional regulator -
H5V41_RS13265 2680857..2681681 - 825 WP_000754763.1 SDR family oxidoreductase -
H5V41_RS13270 2681764..2682018 - 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
H5V41_RS13275 2682015..2682266 - 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
H5V41_RS13280 2682548..2682598 + 51 WP_001364200.1 his operon leader peptide -
H5V41_RS13285 2682744..2683643 + 900 WP_000131776.1 ATP phosphoribosyltransferase -
H5V41_RS13290 2683649..2684953 + 1305 WP_001317939.1 histidinol dehydrogenase -
H5V41_RS13295 2684950..2686020 + 1071 WP_001410397.1 histidinol-phosphate transaminase -
H5V41_RS13300 2686020..2687087 + 1068 WP_000080093.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T172726 WP_000767829.1 NZ_CP061530:c2682018-2681764 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T172726 NZ_CP079891:c1224213-1224058 [Escherichia fergusonii]
ATGAAGCAGCAAAAGGCGATGTTAATCGCCCTGATCGTCATCTGTTTAACCGTCATAGTGACGGCACTGGTAACGAGGAA
AGACCTCTGCGAGGTACGAATCCGAACCGGCCAGACGGAGGTCGCTGTCTTCACAGCTTACGAATCTGAGGAGTAA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT172726 WP_001259255.1 NZ_CP061530:c2682266-2682015 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT172726 NZ_CP079891:1224225-1224283 [Escherichia fergusonii]
CCTTGCCTTTCGGCACGTAAGAGGCTAACCTAGATATGCCGTTCATAGATTGAGCCTCA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References