Detailed information of TA system TA172711

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): ralRA/-
• Location: 1923518..1923889
• Replicon: chromosome
• Accession: NZ_CP061530
• Organism: Escherichia coli strain WEM25

Toxin (Protein)

• Gene name: ralR
• Uniprot ID: F4VC37
• Locus tag: H5V41_RS09360
• Protein ID: WP_001317028.1
• Coordinates: 1923695..1923889 (-)
• Length: 65 a.a.

Antitoxin (RNA)

Gene name	ralA
Locus tag	-
Coordinates	1923518..1923696 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H5V41_RS09335	1919473..1920846	+	1374	WP_000123737.1	ATP-dependent RNA helicase DbpA	-
H5V41_RS09340	1920975..1921910	-	936	WP_001157406.1	tRNA 2-thiocytidine(32) synthetase TtcA	-
H5V41_RS09345	1921962..1923197	-	1236	WP_000040852.1	site-specific integrase	-
H5V41_RS09350	1923199..1923414	-	216	WP_000079604.1	excisionase XisR	-
H5V41_RS09355	1923493..1923702	-	210	WP_000276809.1	double-strand break reduction protein RcbA	-
-	1923518..1923696	+	179	NuclAT_0	-	Antitoxin
-	1923518..1923696	+	179	NuclAT_0	-	Antitoxin
-	1923518..1923696	+	179	NuclAT_0	-	Antitoxin
-	1923518..1923696	+	179	NuclAT_0	-	Antitoxin
H5V41_RS09360	1923695..1923889	-	195	WP_001317028.1	type I toxin-antitoxin system endodeoxyribonuclease toxin RalR	Toxin
H5V41_RS09365	1923946..1924755	-	810	WP_000166319.1	recombination protein RecT	-
H5V41_RS09370	1924748..1927348	-	2601	WP_190812194.1	exodeoxyribonuclease VIII	-
H5V41_RS09375	1927450..1927725	-	276	WP_000632297.1	protein RacC	-
H5V41_RS09380	1927800..1927970	-	171	WP_001352098.1	conserved protein, Rac prophage	-
H5V41_RS09385	1927970..1928191	-	222	WP_000560223.1	killing protein KilR	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1921962..1950479	28517

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Endodeoxyribonuclease toxin RalR (6-54)

Sequences

Toxin

Protein

• Length: 65 a.a.
• Molecular weight: 7018.89 Da
• Isoelectric Point: 8.9538

>T172711 WP_001317028.1 NZ_CP061530:c1923889-1923695 [Escherichia coli]
MRYDNVKPCPFCGCPSVTVKAISGYYRAKCNGCESRTGYGGSEKEALERWNKRTTGNNNGGVHV

Nucleotide

• Length: 195 bp

>T172711 NZ_CP079884:c3622773-3622375 [Escherichia fergusonii]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCCAAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGAGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACA
AATGACGAAGCGCATTTGGTATATTGTCAGGGCGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Nucleotide

• Length: 179 bp

>AT172711 NZ_CP061530:1923518-1923696 [Escherichia coli]
GAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGA
GAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTT
CAATAGTGGCGGTAATTTT

Structures

Toxin

• AlphaFold DB: A0A7L6LHV7

Antitoxin