Detailed information of TA system TA172709

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 809681..810375
• Replicon: chromosome
• Accession: NZ_CP061530
• Organism: Escherichia coli strain WEM25

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: S1EXB8
• Locus tag: H5V41_RS03815
• Protein ID: WP_001263493.1
• Coordinates: 809977..810375 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: -
• Locus tag: H5V41_RS03810
• Protein ID: WP_001399937.1
• Coordinates: 809681..809974 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H5V41_RS03790	805219..805716	+	498	WP_000006260.1	REP-associated tyrosine transposase RayT	-
H5V41_RS03795	806034..807773	-	1740	WP_190812100.1	flagellar biosynthesis protein FlhA	-
H5V41_RS03800	807718..808503	+	786	WP_000207587.1	putative lateral flagellar export/assembly protein LafU	-
H5V41_RS03805	808574..809629	+	1056	WP_001226161.1	DNA polymerase IV	-
H5V41_RS03810	809681..809974	+	294	WP_001399937.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
H5V41_RS03815	809977..810375	+	399	WP_001263493.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
H5V41_RS03820	810385..810837	+	453	WP_001059847.1	GNAT family N-acetyltransferase	-
H5V41_RS03825	811157..811360	+	204	Protein_737	RNA ligase RtcB family protein	-
H5V41_RS03830	811359..811880	+	522	Protein_738	peptide chain release factor H	-
H5V41_RS03835	811937..813394	-	1458	WP_001293003.1	cytosol nonspecific dipeptidase	-
H5V41_RS03840	813655..814113	+	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
-	814709..814789	+	81	NuclAT_11	-	-
-	814709..814789	+	81	NuclAT_11	-	-
-	814709..814789	+	81	NuclAT_11	-	-
-	814709..814789	+	81	NuclAT_11	-	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15472.83 Da
• Isoelectric Point: 8.0949

>T172709 WP_001263493.1 NZ_CP061530:809977-810375 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKQARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T172709 NZ_CP079884:3403536-3403754 [Escherichia fergusonii]
ATGTCCGATAAGCCTTTAACCAAAACTGATTATTTGATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAACGTGT
CATCGAAAAAAATAAATACGAACTCTCTGACAATGAACTGGCGGTATTTTATTCAGCAGCTGATCACCGTCTCGCTGAAT
TGACCATGAATAAACTTTACGACAAGATCCCCTCCTCCGTATGGAAATTCATTCGCTAA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11267.78 Da
• Isoelectric Point: 5.2170

>AT172709 WP_001399937.1 NZ_CP061530:809681-809974 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARFEEITRRA
EQYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT172709 NZ_CP079884:3403135-3403509 [Escherichia fergusonii]
ATGGACGAATACTCACCAAAACGACATGATATCGCACAGCTTAAATTTCTTTGCGAAACCCTGTATCATGACTGTCTTGC
AAATCTTGAAGAGAGCAACCATGGCTGGGTTAACGACCCAACCTCGGCTATCAATCTGCAGCTCAACGAACTGATTGAGC
ATATTGCAACCTTCGCACTTAATTACAAAATTAAGTATAATGAGGACAATAAGCTCATAGAGCAGATCGATGAATATCTG
GATGACACTTTTATGTTGTTCAGTAGTTATGGCATTAATACACAGGATTTACAGAAATGGCGAAAATCTGGAAACAGGCT
CTTCCGCTGTTTTGTTAACGCCACCAGGGCGAACCCTGTTAGTTTGTCGTGTTAA

Structures

Toxin

• AlphaFold DB: S1EXB8