Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/Tad-HTH_37
Location 4009991..4010588 Replicon chromosome
Accession NZ_CP061389
Organism Klebsiella pneumoniae strain NU-CRE195

Toxin (Protein)


Gene name higB Uniprot ID R4YIC5
Locus tag HCV84_RS19640 Protein ID WP_004142563.1
Coordinates 4010271..4010588 (-) Length 106 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID R4YH91
Locus tag HCV84_RS19635 Protein ID WP_004142561.1
Coordinates 4009991..4010278 (-) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HCV84_RS19620 4008115..4008363 + 249 WP_002893037.1 DUF1158 domain-containing protein -
HCV84_RS19625 4008381..4008722 - 342 WP_002893035.1 RamA family antibiotic efflux transcriptional regulator -
HCV84_RS19630 4008753..4009658 - 906 WP_004196121.1 MBL fold metallo-hydrolase -
HCV84_RS19635 4009991..4010278 - 288 WP_004142561.1 helix-turn-helix transcriptional regulator Antitoxin
HCV84_RS19640 4010271..4010588 - 318 WP_004142563.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
HCV84_RS19645 4010773..4011816 - 1044 WP_004178895.1 DUF2157 domain-containing protein -
HCV84_RS19650 4012483..4013349 - 867 WP_004178894.1 helix-turn-helix transcriptional regulator -
HCV84_RS19655 4013458..4014885 + 1428 WP_004176980.1 MFS transporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-100)

Antitoxin

Cro/C1-type helix-turn-helix domain

(38-88)


Sequences

Toxin        


Download         Length: 106 a.a.        Molecular weight: 12110.39 Da        Isoelectric Point: 11.2767

>T172583 WP_004142563.1 NZ_CP061389:c4010588-4010271 [Klebsiella pneumoniae]
MFRLVVHVDVKKELQALPPIVQAKMIRQLDKLRQNPTALREPDSKPLGQGLFEIRALGSVQGRGIYVFQQGKTLFLLRVF
VKKTQKTPSSEIRLALKRLEEMRNE

Download         Length: 318 bp

>T172583 NZ_CP079796:c578-315 [Acinetobacter johnsonii]
ATGCATGTATTTACATATACGGACGCACGTAACAATTTAAAATCTGTTTTAGATAAAGTAATTGATGATGTAGACGTTGC
TTTTATTACAAGAAAAGAAGGTGGCCATGCTGTAGTAATGGGACAAGATCATTATGATAGCTTAATGGAAACGTTATATC
TCCTTTCATCTCCAAATAATGCAAGTCGTTTAAATGAATCAATTGCTCAATTACGTGCAGGGAAAACAAAACACAGAGAG
TTGATTGAGGATGACGAATCGTAA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10771.46 Da        Isoelectric Point: 10.2212

>AT172583 WP_004142561.1 NZ_CP061389:c4010278-4009991 [Klebsiella pneumoniae]
MSKKIIDWDTLKTELLGQPEVQAAFDAQERQQRLRTMLAQWREQAGLTRAQVAQRMGVSAPTVSRMEANITRASLDTLTR
YALACGIKHPQVLLY

Download         Length: 288 bp

>AT172583 NZ_CP079796:c328-65 [Acinetobacter johnsonii]
ATGACGAATCGTAACGTCGAATGGACTGATAATGCCTGGGATGAATATATCTATTGGCAGACACAGGATAAAAAGATACT
TAAGCGTATTAATACCTTAATCAAAGAATGTCAGCGAACACCTTTTGAAGGAACAGGAAAACCAGAACCTTTAAAAGCTA
ATCTTTCAGGATTTTGGAGTCGTAGGATTGATGAAAAGCATAGATTAGTTTATGAAGTGACAGATGAACGAATCTCTATA
ATTCAATGTCGATTCCATTACTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0M5MXH8

Antitoxin

Source ID Structure
AlphaFold DB A0A0S3DIQ1

References