Detailed information of TA system TA172522

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-VapI
• Location: 4602657..4603274
• Replicon: chromosome
• Accession: NZ_CP061378
• Organism: Bradyrhizobium genosp. L strain BDV5419

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: IC762_RS21820
• Protein ID: WP_195784293.1
• Coordinates: 4602990..4603274 (-)
• Length: 95 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: IC762_RS21815
• Protein ID: WP_195784292.1
• Coordinates: 4602657..4602974 (-)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IC762_RS21790	4598034..4598486	+	453	WP_195784289.1	hypothetical protein	-
IC762_RS21795	4598523..4599494	-	972	WP_195784290.1	amidohydrolase family protein	-
IC762_RS21800	4599674..4600132	+	459	WP_195790232.1	GNAT family N-acetyltransferase	-
IC762_RS21805	4600145..4601365	-	1221	WP_195784291.1	amidohydrolase family protein	-
IC762_RS21810	4601467..4602585	-	1119	Protein_4327	RluA family pseudouridine synthase	-
IC762_RS21815	4602657..4602974	-	318	WP_195784292.1	HigA family addiction module antidote protein	Antitoxin
IC762_RS21820	4602990..4603274	-	285	WP_195784293.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
IC762_RS21825	4603434..4604102	-	669	WP_195784294.1	hypothetical protein	-
IC762_RS21830	4604158..4605489	-	1332	WP_195784295.1	replication-associated recombination protein A	-
IC762_RS21835	4605486..4606898	-	1413	WP_195784296.1	DegQ family serine endoprotease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (6-94)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (3-80)

Sequences

Toxin

Protein

• Length: 95 a.a.
• Molecular weight: 10517.90 Da
• Isoelectric Point: 6.7178

>T172522 WP_195784293.1 NZ_CP061378:c4603274-4602990 [Bradyrhizobium genosp. L]
VIVGHRDKVTERFASGLHVKEFSGFARQAEMRLDRLEAAASLADLAALPGNRLEALKGDRAGQFSIRINDQWRICFRWPD
GSPGPVDVEIVDYH

Nucleotide

• Length: 285 bp

>T172522 NZ_CP079773:28833-29210 [Escherichia coli]
ATGAGGCATATATCACCGGAAGAGCTTATTGCAATTCATGATGCGAATATAAGCCGCTACGGCGGCCTGCCAGGCATGCC
AGATTCGGGAAGAGCTGAGGCTATTATCGGGAGAGTCCAGGCCAGAGTTGCATATGAAGAGATTACCGACCTTTTCGAAG
TCTCCGCAACTTACCTGGTGGCTACTGCGAGAGGGCATATATTCAATGATGCCAATAAGCGTACCGCGCTAAACAGTGCG
TTGTTATTTCTGCGCCGTAACGGTGTGCAGGTATATGATTCACCAGAACTGGCAGAGCTTACTGTGGGGGCTGCGACCGG
AGAGGTATCCGTATCTACTGTCGTTGCCACGTTACGTAGATTGTATGGTACGGCGTAG

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 11726.52 Da
• Isoelectric Point: 10.7753

>AT172522 WP_195784292.1 NZ_CP061378:c4602974-4602657 [Bradyrhizobium genosp. L]
MPRTPIHPGEHLAEELRELGMTAAELSRQIDVPVNRITGIIHGQRGITADTALRLGHWFGTTPQFWMNLQQLYELRLAEN
EVGVQIAALPRRAGRPSPRKLGKTA

Nucleotide

• Length: 318 bp

>AT172522 NZ_CP079773:28612-28833 [Escherichia coli]
ATGCAATCTATTAACTTCCGTACCGCACGCAGCAATCTTACCGAAGTGCTCAACAATGTTGAAGCCGGAGAAGAGGTTGA
AATCACCCGTAGAGGCCGTGAGCCAGCTGTAATTGTCAGCAAGGCCACTTTCGAAGCCTACAAAAAAGCGGCGCTGGATG
CTGAATTTGCATCCCTGTTTGACACTCTGGACACCACCAATAAGAAACTGGTTAACCGATAA