Detailed information of TA system TA172520

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 5358616..5359211
• Replicon: chromosome
• Accession: NZ_CP061376
• Organism: Pseudomonas aeruginosa strain HS17-127

Toxin (Protein)

• Gene name: higB
• Uniprot ID: V6ALY3
• Locus tag: ICI41_RS24960
• Protein ID: WP_003113526.1
• Coordinates: 5358933..5359211 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: ICI41_RS24955
• Protein ID: WP_003133769.1
• Coordinates: 5358616..5358921 (-)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ICI41_RS24925 (ICI41_24920)	5354222..5354557	+	336	WP_025921265.1	hypothetical protein	-
ICI41_RS24930 (ICI41_24925)	5354567..5354956	+	390	WP_025921264.1	(deoxy)nucleoside triphosphate pyrophosphohydrolase	-
ICI41_RS24935 (ICI41_24930)	5355036..5355767	+	732	WP_124132542.1	HNH endonuclease	-
ICI41_RS24940 (ICI41_24935)	5355847..5356830	-	984	WP_121499923.1	anti-phage protein KwaB	-
ICI41_RS24945 (ICI41_24940)	5356827..5357417	-	591	WP_124132543.1	anti-phage protein KwaA	-
ICI41_RS24950 (ICI41_24945)	5357867..5358280	-	414	WP_121499921.1	hypothetical protein	-
ICI41_RS24955 (ICI41_24950)	5358616..5358921	-	306	WP_003133769.1	HigA family addiction module antitoxin	Antitoxin
ICI41_RS24960 (ICI41_24955)	5358933..5359211	-	279	WP_003113526.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
ICI41_RS32520	5359264..5359392	-	129	Protein_4941	integrase	-
ICI41_RS24965 (ICI41_24960)	5359540..5361768	+	2229	WP_043089846.1	TonB-dependent receptor	-
ICI41_RS24970 (ICI41_24965)	5361838..5362485	-	648	WP_003095021.1	carbonate dehydratase	-
ICI41_RS24975 (ICI41_24970)	5362547..5363785	-	1239	WP_014603324.1	C69 family dipeptidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (5-92)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (6-82)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10614.13 Da
• Isoelectric Point: 7.8937

>T172520 WP_003113526.1 NZ_CP061376:c5359211-5358933 [Pseudomonas aeruginosa]
VILTFRCDETRQLFETGLSRRWGAILTVATRKLAMLHAATELRDLRSPPGNRLEPLQGKRAGQHSIRINDQWRVCFVWTD
AGPEEVEIVDYH

Nucleotide

• Length: 279 bp

>T172520 NZ_CP079772:4363924-4364331 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCACTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCTGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11214.73 Da
• Isoelectric Point: 4.6081

>AT172520 WP_003133769.1 NZ_CP061376:c5358921-5358616 [Pseudomonas aeruginosa]
MATNGMRPIHPGEILRDEFLMELDISPAALARALKVSAPTVNDIVREQRGISADMAIRLGRYFDTSAQFWMNLQSEYSLA
TAYAANGEQIEHEIEPLLAHG

Nucleotide

• Length: 306 bp

>AT172520 NZ_CP079772:4363677-4363943 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Structures

Toxin

• AlphaFold DB: V6ALY3