Detailed information of TA system TA172496

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 1689004..1689551
• Replicon: chromosome
• Accession: NZ_CP061368
• Organism: Wenzhouxiangella sp. AB-CW3

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: IC757_RS07330
• Protein ID: WP_190976683.1
• Coordinates: 1689297..1689551 (-)
• Length: 85 a.a.

Antitoxin (Protein)

• Gene name: graA
• Uniprot ID: -
• Locus tag: IC757_RS07325
• Protein ID: WP_190976682.1
• Coordinates: 1689004..1689297 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IC757_RS07305	1684347..1684778	+	432	WP_190976679.1	hypothetical protein	-
IC757_RS07310	1684848..1687532	+	2685	WP_190976680.1	tetratricopeptide repeat protein	-
IC757_RS07315	1687572..1688168	+	597	WP_190976681.1	sigma-70 family RNA polymerase sigma factor	-
IC757_RS07320	1688352..1688762	-	411	WP_190976971.1	GNAT family N-acetyltransferase	-
IC757_RS07325	1689004..1689297	-	294	WP_190976682.1	HigA family addiction module antidote protein	Antitoxin
IC757_RS07330	1689297..1689551	-	255	WP_190976683.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
IC757_RS07335	1690025..1690396	-	372	WP_190976684.1	hypothetical protein	-
IC757_RS07340	1690612..1691253	+	642	Protein_1435	IS4 family transposase	-
IC757_RS07345	1691384..1692910	+	1527	WP_190976685.1	IS21 family transposase	-
IC757_RS07350	1692897..1693637	+	741	WP_190974040.1	IS21-like element helper ATPase IstB	-
IC757_RS07355	1693700..1694233	+	534	Protein_1438	IS4 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (2-84)

Antitoxin

• Cro/C1-type helix-turn-helix domain (18-66)

Sequences

Toxin

Protein

• Length: 85 a.a.
• Molecular weight: 9679.94 Da
• Isoelectric Point: 6.8783

>T172496 WP_190976683.1 NZ_CP061368:c1689551-1689297 [Wenzhouxiangella sp. AB-CW3]
VLAKFYETGSVAGVQPSHAKRLRMLLAALDTAASIQDMDVPGFRLHRLKGMDADRWSIRVSENWRLTYEFRDGQAHVVDY
EDYH

Nucleotide

• Length: 255 bp

>T172496 NZ_CP079770:4146726-4147103 [Escherichia coli]
ATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCGGTTCACGCCCGTCTCCGGTTGAAATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCATTACGGCCTCACGCTGAATGACACACCGTTCGCCGATGAACGTGTGATTGAGCAGCATATTG
AAGCTGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTTGAAAAATACGCGCTGGTACGTACCGACCAGCCGGGATTC
AGCACCTGTACCCACTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCCCGCCGGGCGACAGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATGGCATTACCCAGGGGAAGCATCCGGAGGCGAAACAATGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11117.73 Da
• Isoelectric Point: 6.8705

>AT172496 WP_190976682.1 NZ_CP061368:c1689297-1689004 [Wenzhouxiangella sp. AB-CW3]
MSMHNPPHPGEFIREVYLEPHSMTGRQLASKLCVSPSTLNRILQERSGISPEMALRLSKALGRSPESWLLMQDNYELWHA
RQTVDLESVEKVEFRAA

Nucleotide

• Length: 294 bp

>AT172496 NZ_CP079770:4146268-4146636 [Escherichia coli]
GTGTCAGACACGCTCCATGAAACAAATTATCCCGACGACAACAACGACCGCCCCTGGTGGGGACTCCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAAGACAACCAGTTGCATTACCTTGCCGACCGTGCCGGTATCAGAGGCCGGT
TCAGCGACGCGGATGCGTACCACCTGGACCAGGCCCTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAGGGCTGACCTGCGAAGCCGACACCCTCGGCAGTTG
TGGCTACGTTTATATGGCTGTTTATCCGACGCCCGAAACGAAAAAGTAA