Detailed information of TA system TA172478

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 1888033..1888760
• Replicon: chromosome
• Accession: NZ_CP061367
• Organism: Shigella sonnei strain 6207

Toxin (Protein)

• Gene name: higB
• Uniprot ID: Q3YYE6
• Locus tag: IC759_RS10185
• Protein ID: WP_000547563.1
• Coordinates: 1888033..1888344 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: IC759_RS10190
• Protein ID: WP_000126296.1
• Coordinates: 1888341..1888760 (+)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IC759_RS10155 (1883187)	1883187..1884884	+	1698	WP_001288143.1	formate hydrogenlyase subunit HycE	-
IC759_RS10160 (1884894)	1884894..1885436	+	543	WP_000493785.1	formate hydrogenlyase subunit HycF	-
IC759_RS10165 (1885436)	1885436..1886203	+	768	WP_000067403.1	formate hydrogenlyase subunit HycG	-
IC759_RS10170 (1886200)	1886200..1886610	+	411	WP_001291918.1	formate hydrogenlyase assembly protein HycH	-
IC759_RS10175 (1886603)	1886603..1887073	+	471	WP_000132961.1	hydrogenase maturation peptidase HycI	-
IC759_RS10180 (1887098)	1887098..1887871	+	774	WP_001026444.1	hypothetical protein	-
IC759_RS10185 (1888033)	1888033..1888344	+	312	WP_000547563.1	type II toxin-antitoxin system HigB family toxin	Toxin
IC759_RS10190 (1888341)	1888341..1888760	+	420	WP_000126296.1	helix-turn-helix domain-containing protein	Antitoxin
IC759_RS10195 (1888874)	1888874..1890298	-	1425	WP_000110319.1	6-phospho-beta-glucosidase AscB	-
IC759_RS10200 (1890307)	1890307..1891764	-	1458	WP_001107890.1	PTS cellobiose/arbutin/salicin transporter subunit IIBC	-
IC759_RS10205 (1892024)	1892024..1893034	+	1011	WP_001402444.1	DNA-binding transcriptional regulator AscG	-
IC759_RS10210 (1893182)	1893182..1893709	+	528	WP_001078777.1	electron transport protein HydN	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12398.11 Da
• Isoelectric Point: 9.7105

>T172478 WP_000547563.1 NZ_CP061367:1888033-1888344 [Shigella sonnei]
MHIISKAPFEESARKYPNDALALQALYRVIKETDFSTPEEMRTAFPNLDNFKYRNKWYVLDVGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T172478 NZ_CP079767:4441337-4441672 [Escherichia coli]
ATGGTAAGCCGATACGTACCCGATATGGGCGATCTGATTTGGGTTGATTTTGACCCGACAAAAGGTAGCGAGCAAGCTGG
ACATCGTCCAGCTGTTGTCCTGAGTCCTTTCATGTACAACAACAAAACAGGTATGTGTCTGTGTGTTCCTTGTACAACGC
AATCAAAAGGATATCCGTTCGAAGTTGTTTTATCCGGTCAGGAACGTGATGGCGTAGCGTTAGCTGATCAGGTAAAAAGT
ATCGCCTGGCGGGCAAGAGGAGCAACGAAGAAAGGAACAGTTGCCCCAGAGGAATTACAACTCATTAAAGCCAAAATTAA
CGTACTGATTGGGTAG

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15416.38 Da
• Isoelectric Point: 4.3653

>AT172478 WP_000126296.1 NZ_CP061367:1888341-1888760 [Shigella sonnei]
MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLASRIAEYENNNQEFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT172478 NZ_CP079767:4441089-4441337 [Escherichia coli]
ATGATCCACAGTAGCGTAAAGCGTTGGGGAAATTCACCGGCGGTGCGGATCCCGGCTACGTTAATGCAGGCGCTCAATCT
GAATATTGATGATGAAGTGAAGATTGACCTGGTGGATGGCAAATTAATTATTGAGCCAGTGCGTAAAGAGCCCGTATTTA
CGCTTGCTGAACTGGTCAACGACATCACGCCGGAAAACCTCCACGAGAATATCGACTGGGGAGAGCCGAAAGATAAGGAA
GTCTGGTAA

Structures

Toxin

• AlphaFold DB: A0A7U9QA22