Detailed information of TA system TA172446

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yefM-yoeB (relBE)/Txe-RelB
• Location: 2451257..2451774
• Replicon: chromosome
• Accession: NZ_CP061349
• Organism: Staphylococcus aureus strain MSSA01

Toxin (Protein)

• Gene name: yoeB
• Uniprot ID: T1YC79
• Locus tag: IC588_RS12225
• Protein ID: WP_000113262.1
• Coordinates: 2451257..2451523 (-)
• Length: 89 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: T1YB60
• Locus tag: IC588_RS12230
• Protein ID: WP_000584499.1
• Coordinates: 2451523..2451774 (-)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IC588_RS12200	2446680..2447216	-	537	WP_196584239.1	GNAT family N-acetyltransferase	-
IC588_RS12205	2447450..2448274	-	825	WP_000572040.1	formate/nitrite transporter family protein	-
IC588_RS12210	2448491..2448673	-	183	WP_000079142.1	hypothetical protein	-
IC588_RS12215	2448760..2449227	-	468	WP_000153344.1	SRPBCC domain-containing protein	-
IC588_RS12220	2449413..2450963	-	1551	WP_042909117.1	zinc ABC transporter substrate-binding lipoprotein AdcA	-
IC588_RS12225	2451257..2451523	-	267	WP_000113262.1	Txe/YoeB family addiction module toxin	Toxin
IC588_RS12230	2451523..2451774	-	252	WP_000584499.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
IC588_RS12235	2451939..2452040	-	102	Protein_2364	hypothetical protein	-
IC588_RS12240	2452047..2452646	-	600	WP_000162810.1	DsbA family protein	-
IC588_RS12245	2452665..2453027	-	363	WP_000819843.1	DUF4467 domain-containing protein	-
IC588_RS12250	2453282..2454533	+	1252	Protein_2367	FemA/FemB family glycyltransferase FmhA	-
IC588_RS12255	2454630..2455361	-	732	WP_042909118.1	amino acid ABC transporter ATP-binding protein	-
IC588_RS12260	2455358..2456077	-	720	WP_000479576.1	amino acid ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (5-88)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-74)

Sequences

Toxin

Protein

• Length: 89 a.a.
• Molecular weight: 10437.95 Da
• Isoelectric Point: 9.9143

>T172446 WP_000113262.1 NZ_CP061349:c2451523-2451257 [Staphylococcus aureus]
MARLNITFSPQAFEDYKYFQQNDKKMVKKINELLKSIDRNGALEGIGKPEKLKSNLTGYYSRRINHEHRLVYTVDDNHIK
IASCKYHY

Nucleotide

• Length: 267 bp

>T172446 NZ_CP079762:c2867085-2866744 [Klebsiella pneumoniae]
ATGAAAACTCTACCTGCTATAATTTCGCGGGCGGCGAAGCCCTGTCTGTCGCCCGTTGCAGTCTGGCAAATGCTACTTAC
ACGCCTGCTGGAAAAACACTATGGTCTGACCCTGAACGATACGCCGTTCTGTGATGAAACCGTTATACAGGAACATATCG
ATGCCGGTATTACGCTGGCTGATGCCGTTAATTTTCTGGTGGAAAAATACGGACTGGTTCGTATCGACAGGAATGGATTT
GTCTGGCAGGAACAATCACCTTATCTTCGGGCCGTTGATATTCTGCGAGCGCGGCAGGCAACTGGCTTATTGCGACAAAG
CCGTAACAACGCAATACGTTGA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9433.60 Da
• Isoelectric Point: 4.3407

>AT172446 WP_000584499.1 NZ_CP061349:c2451774-2451523 [Staphylococcus aureus]
MIIKNYSYARQNLKALMTKVNDDSDMVTVTSTDDKNVVIMSESDYNSMMETLYLQQNPNNAEHLAQSIADLERGKTITKD
IDV

Nucleotide

• Length: 252 bp

>AT172446 NZ_CP079762:c2867423-2867106 [Klebsiella pneumoniae]
ATGAGCAACATTACATGGGGCCTGCAGCGGGATACCACGCCGCGTCTGGGAGCCCGTCTGGTACAGGAGGGGAACCAACT
GCATTATCTGGCTGACCGGGCCAGTATGACCGGTAAATTCAGTGACGCCGAATGCCGGAAGCTGGATGAAACATTCCCGC
ACTTTATCAGCCAGATGGAATCGATGCTGATCACTGGTGAAATGAATCCTCGCCATGCCCACTGTGTCACCCTGTACCAC
AACGGTTTTACCTGCGAAGCCGATACCCTTGGCAGTTGCGGCTACGTATACATCGCCGTTTACCCCATCCAACGATAA

Structures

Toxin

• PDB: 6L8G
• PDB: 6L8F
• PDB: 7CUA
• PDB: 6L8E

Antitoxin

• PDB: 6L8G
• PDB: 6L8F
• PDB: 7BWF
• PDB: 6L8E
• AlphaFold DB: T1YB60