Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 4035739..4036538 Replicon chromosome
Accession NZ_CP061337
Organism Escherichia coli strain M11957

Toxin (Protein)


Gene name yhaV Uniprot ID B7NDB6
Locus tag IC756_RS19310 Protein ID WP_000347269.1
Coordinates 4036074..4036538 (+) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID V0YUB5
Locus tag IC756_RS19305 Protein ID WP_001309780.1
Coordinates 4035739..4036074 (+) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IC756_RS19290 4031524..4032294 - 771 WP_001058227.1 2-dehydro-3-deoxyglucarate aldolase -
IC756_RS19295 4032310..4033644 - 1335 WP_000599651.1 galactarate/glucarate/glycerate transporter GarP -
IC756_RS19300 4034019..4035590 + 1572 WP_001273738.1 galactarate dehydratase -
IC756_RS19305 4035739..4036074 + 336 WP_001309780.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
IC756_RS19310 4036074..4036538 + 465 WP_000347269.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
IC756_RS19315 4036593..4037402 - 810 WP_000072171.1 aga operon transcriptional regulator AgaR -
IC756_RS19320 4037651..4038931 + 1281 WP_000681943.1 tagatose-bisphosphate aldolase subunit KbaZ -
IC756_RS19325 4038954..4039427 + 474 WP_001295547.1 PTS N-acetylgalactosamine transporter subunit IIB -
IC756_RS19330 4039438..4040217 + 780 WP_000406214.1 PTS mannose/fructose/sorbose/N-acetylgalactosamine transporter subunit IIC -
IC756_RS19335 4040207..4041085 + 879 WP_001295548.1 PTS system mannose/fructose/sorbose family transporter subunit IID -
IC756_RS19340 4041103..4041537 + 435 WP_000948818.1 PTS sugar transporter subunit IIA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 4026497..4036538 10041


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17806.22 Da        Isoelectric Point: 9.6924

>T172401 WP_000347269.1 NZ_CP061337:4036074-4036538 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTREAEETH

Download         Length: 465 bp

>T172401 NZ_CP079747:3521609-3521887 [Escherichia coli]
ATGATTCAAAGGGATATTGAATACTCGGGACAATTTTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATACTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAA
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTATTTGGGTAA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12345.94 Da        Isoelectric Point: 4.8616

>AT172401 WP_001309780.1 NZ_CP061337:4035739-4036074 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNT
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT172401 NZ_CP079747:3521346-3521606 [Escherichia coli]
ATGGCTGCTAACGCGTTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCGGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAAGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATTAAAAACAGCGAAGCTGGCGTTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829IY86

Antitoxin

Source ID Structure
AlphaFold DB V0YUB5

References