Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 3992763..3993490 Replicon chromosome
Accession NZ_CP061337
Organism Escherichia coli strain M11957

Toxin (Protein)


Gene name higB Uniprot ID U9ZMR4
Locus tag IC756_RS19090 Protein ID WP_000550189.1
Coordinates 3993176..3993490 (-) Length 105 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID D3GWQ0
Locus tag IC756_RS19085 Protein ID WP_000560253.1
Coordinates 3992763..3993179 (-) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IC756_RS19075 3988178..3990529 + 2352 WP_000695439.1 alpha-glucosidase -
IC756_RS19080 3990700..3992718 + 2019 WP_000121438.1 NADPH-dependent 2,4-dienoyl-CoA reductase -
IC756_RS19085 3992763..3993179 - 417 WP_000560253.1 type II toxin-antitoxin system antitoxin HigA Antitoxin
IC756_RS19090 3993176..3993490 - 315 WP_000550189.1 type II toxin-antitoxin system toxin HigB Toxin
IC756_RS19095 3993775..3994911 - 1137 WP_000018677.1 23S rRNA (guanine(1835)-N(2))-methyltransferase RlmG -
IC756_RS19100 3994996..3995499 + 504 WP_001309773.1 M48 family metallopeptidase -
IC756_RS19105 3995576..3996259 + 684 WP_001183054.1 vancomycin high temperature exclusion protein -
IC756_RS19110 3996338..3997324 + 987 WP_001377074.1 Gfo/Idh/MocA family oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(20-92)

Antitoxin

Antitoxin HigA

(1-138)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 12103.10 Da        Isoelectric Point: 10.0409

>T172400 WP_000550189.1 NZ_CP061337:c3993490-3993176 [Escherichia coli]
MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAVFPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKC
YIREVMTHKEYDFFTAVHRTKGKK

Download         Length: 315 bp

>T172400 NZ_CP079747:c3515555-3515157 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGTGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACAGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 14965.39 Da        Isoelectric Point: 4.4547

>AT172400 WP_000560253.1 NZ_CP061337:c3993179-3992763 [Escherichia coli]
MIAIADILQAGEKLTAVAPFLAGIQNEEQYAQALELVDHLLLNDPENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGG
IAVIRTLMDQYGLTLSDLPEIGSKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID

Download         Length: 417 bp

>AT172400 NZ_CP079747:c3515851-3515558 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATTACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGAGCT
GAAAAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6KMQ
PDB 5IFG
PDB 5YCL
PDB 6KML

Antitoxin

Source ID Structure
AlphaFold DB A0A829IXD3

References