Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 2184139..2184701 Replicon chromosome
Accession NZ_CP061337
Organism Escherichia coli strain M11957

Toxin (Protein)


Gene name higB Uniprot ID L4J948
Locus tag IC756_RS10460 Protein ID WP_000605676.1
Coordinates 2184423..2184701 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID S1PAQ1
Locus tag IC756_RS10455 Protein ID WP_000781370.1
Coordinates 2184139..2184423 (-) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IC756_RS10440 2179321..2182368 + 3048 WP_012602782.1 formate dehydrogenase-N subunit alpha -
IC756_RS10445 2182381..2183265 + 885 WP_001240565.1 formate dehydrogenase N subunit beta -
IC756_RS10450 2183258..2183911 + 654 WP_000045646.1 formate dehydrogenase-N subunit gamma -
IC756_RS10455 2184139..2184423 - 285 WP_000781370.1 HigA family addiction module antidote protein Antitoxin
IC756_RS10460 2184423..2184701 - 279 WP_000605676.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
IC756_RS10465 2184887..2185897 - 1011 WP_000642407.1 alcohol dehydrogenase AdhP -
IC756_RS10470 2186031..2187728 - 1698 WP_000433464.1 malate dehydrogenase -
IC756_RS10475 2187885..2188022 - 138 WP_000841554.1 stationary-phase-induced ribosome-associated protein -
IC756_RS10480 2188124..2188339 - 216 WP_000495766.1 biofilm-dependent modulation protein -
IC756_RS10485 2188684..2189115 + 432 WP_000152305.1 peroxiredoxin OsmC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(4-92)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(1-88)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10521.10 Da        Isoelectric Point: 7.3562

>T172388 WP_000605676.1 NZ_CP061337:c2184701-2184423 [Escherichia coli]
MIMNFRHKGLRDLFLLGKTSGVIPTQVKRLRHRLAVIDAASCLADIDMPGYRLHPLSGDRDGIWAISVSGNWRITFEFVN
GDAYILDYEDYH

Download         Length: 279 bp

>T172388 NZ_CP079747:211261-211368 [Escherichia coli]
ATGACGTTCGCAGAGCTGGGCATGGCCTTCTGGCATGATTTAGCGGCTCCGGTCATTGCTGGCATTCTTGCCAGTATGAT
CGTGAACTGGCTGAACAAGCGGAAGTAA

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10401.08 Da        Isoelectric Point: 8.8567

>AT172388 WP_000781370.1 NZ_CP061337:c2184423-2184139 [Escherichia coli]
MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTGKAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAE
KTVDVSRLRRLVTQ

Download         Length: 285 bp

>AT172388 NZ_CP079747:c211213-211147 [Escherichia coli]
GGTCTAGAGTCAAGATTAGCCCCCGTGGTGTTGTCAGGTGCATACCTGCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829G6T1

Antitoxin

Source ID Structure
PDB 2ICT
PDB 2ICP
AlphaFold DB A0A829CUG6

References